FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482697
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14490.3001883168944493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACT306.551335E-445.647714
TTAGTAT350.001177112940.52458663
GTTAGTA458.579049E-539.3482462
GTACTAT350.001398207739.130661
CATATAA350.0013989239.1266062
ATATAAC350.0013989239.1266063
GTATCAA16900.033.226331
GGTATCA8350.032.804151
TGTTAGT552.8127898E-432.1526761
GTGGTAT3500.031.304531
ACTTAAG450.00429425831.12834752
TAAGTAA550.00503252630.13385669
TGGTATC3750.030.1274872
TATCAAC19600.028.6462672
AACGCAG19500.028.0908976
TCAACGC20050.028.003334
CAACGCA20250.027.5576925
ATCAACG20300.027.4898133
AGAGTAC20300.027.00904811
CAGAGTA20550.026.84722510
Produced by FastQC (version 0.11.5)