FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195591
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27790.23243734688534792No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24940.20859976363154287No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24230.2026612779788406No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGTATTAATTGGAATAGTTAT22800.19070066603044017No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21860.1828384455888343No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19590.1638520196287861No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18590.15548795532920537No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCT18490.15465154889924732No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGACT18440.15423334568426827No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18350.153480579897306No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17850.14929854774751566No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17600.1472075316726205No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17460.1460365626706792No Hit
TCCCTGGACCTTGCCGGACCCAGTGCATGGCATGATCTTCAAAAGTGAAT17280.14453103109675466No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17070.1427745775938427No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGATGGATTTTC16080.1344941539372578No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAGTTACTTATCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT16060.1343268726512662No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15830.13240313786236263No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15830.13240313786236263No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15630.13073032500244647No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15310.12805382442658067No Hit
GTCTGAGCCCTGACGACACGGCCTTGTATTTCTGTACAAGAGGGTCCGGTGGGGGGGCCCTACGACATATTGACT15200.12713377735362677No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15130.12654829285265612No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14030.11734782212311735No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13610.11383491511729345No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13590.11366763383130184No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAAGTG13410.11216210225737731No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCAGGGGCCCTGTT13290.11115841454142762No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13170.11015472682547796No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13170.11015472682547796No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13090.10948560168151147No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13000.10873283589454923No Hit
GTGTTAGGAGTTACTTATCCTGGTACCAACAGAAACCTGGCCAGGCTCCC12750.10664181981965405No Hit
GTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGAGTTACTTATCCTGGTA12280.10271070959885112No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12240.10237614702686788No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12030.10061969352395594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.048.480451
GTATCAA22950.045.0282821
TATCAAC25400.040.2629932
ATCAACG27350.037.3923233
TCAACGC27650.036.9866184
CAACGCA27650.036.737555
AACGCAG28200.036.0210386
ACGAGAC1450.033.25186522
AGAGTAC31150.032.72027611
GCAGAGT31950.032.0087669
CGCAGAG31900.031.843058
ACGCAGA32400.031.3516467
TATACAC1800.030.6077063
CAGAGTA33550.030.37963310
CTTATAC1900.029.0076941
CTCGTAT1352.9467628E-1028.21044239
TCGTATG1503.274181E-1127.70567740
TTATACA1800.026.7817422
GAGTACT10300.026.74459812
AGTACTT10500.026.23517613
Produced by FastQC (version 0.11.5)