FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195591
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28410.23762306675108794No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26630.22273503229783428No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22030.18426033651976303No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21300.1781545695810691No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGTATTAATTGGAATAGTTAT20680.17296884971532908No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19600.1639356602717819No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGACT18480.1545679082562515No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17450.1459529220276834No Hit
TCCCTGGACCTTGCCGGACCCAGTGCATGGCATGATCTTCAAAAGTGAAT17200.14386190595278822No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCT16990.14210545244987624No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16580.13867618608704818No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16040.13415959136527458No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15640.1308139656454423No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15210.1272174179966226No Hit
GTCTGAGCCCTGACGACACGGCCTTGTATTTCTGTACAAGAGGGTCCGGTGGGGGGGCCCTACGACATATTGACT14840.12412271420577774No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14740.12328630777581967No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14720.12311902648982805No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14630.12236626070286577No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAGTTACTTATCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT14460.12094436977193705No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14330.11985704141299156No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14310.11968976012699994No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13950.1166786969791509No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13870.11600957183518444No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13380.1119111803283899No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAAGTG13360.11174389904239827No Hit
GTGTTAGGAGTTACTTATCCTGGTACCAACAGAAACCTGGCCAGGCTCCC13220.11057293004045698No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13090.10948560168151147No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGATGGATTTTC13050.10915103910952827No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13010.10881647653754503No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCAGGGGCCCTGTT13000.10873283589454923No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12760.10672546046264986No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12670.10597269467568758No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12510.10463444438775467No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT12220.10220886574087626No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12110.10128881866792239No Hit
CTCCAGGCCCTTCCCTGGACCTTGCCGGACCCAGTGCATGGCATGATCTT12050.10078697480994755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)