FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103073
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC66980.607212759264346No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA44070.3995202493397989No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT42740.3874630237527344No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC40630.36833464331009824No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGATTACCA40630.36833464331009824No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC36960.33506395315631876No Hit
CCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCT36890.33442936233594694No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34510.3128532744433052No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32060.29064259573029166No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGATTACCAC31080.28175832424508623No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA30600.27740684433396523No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC30330.2749591368839596No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTT29960.27160487111913717No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG29180.26453371626356553No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC29170.2644430604320838No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG28870.2617233854876332No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27540.2496661599005687No Hit
GTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAG26160.23715565515609574No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGG25630.23235089608756626No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25230.22872466282829873No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG24660.22355728043384257No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24090.21838989803938635No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23660.21449169728567377No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22960.20814578908195558No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC22280.2019811925412008No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGATTAC21960.19908020593378678No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA21730.19699512180970796No Hit
GTAGTAGTCAGCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGTGG21440.194366102696739No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20460.1854818312115336No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20220.18330609125597308No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATTATGTCTTCGG19960.1809490396374492No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTCTTGGTACCAGCAGAAG19100.17315263813002402No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19050.1726993589726156No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18850.17088624234298183No Hit
CTACTACTGTTACTCAACAGACAGCAGTGGTAATCATTATGTCTTCGGAA18690.16943574903927483No Hit
CAGTAGTAGTCAGCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGT18550.1681665673985312No Hit
GCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCGGGTGGAGGATGAAGCTGACTACTACTGTTACTCAA18480.16753197657815938No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTC18220.16517492495963548No Hit
GTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT18220.16517492495963548No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC18190.16490295746519043No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18160.16463098997074535No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATT17620.15973557507073421No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17500.15864770509295398No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTCTTGGTACCAGCAGAAGTCAGGCCAGGCCC17370.157469179283692No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17250.15638130930591176No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA17120.15520278349664982No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16620.15066999192256542No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16440.14903818695589502No Hit
TCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC16210.1469531028318162No Hit
GTAATACATAGCCGTGTCCTCAGCTCTCAGGCTGTTCGTTTGCAGATACA15900.14414277205588388No Hit
GACTATCAGTGGGGCCCGGGTGGAGGATGAAGCTGACTACTACTGTTACT15180.13761555218920235No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15040.13634637054845872No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC14600.13235751396326445No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14590.13226685813178277No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14580.13217620230030108No Hit
GCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCC14420.13072570899659405No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14410.13063505316511237No Hit
GCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCC14390.130453741502149No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG14240.12909390402992366No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13450.12193209334287033No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT13360.12111619085953514No Hit
GGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC13290.12048160003916332No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13240.12002832088175489No Hit
ATGTATTACTGTGCGAGAGAGCCATCTTGTGGTGGTGACTGCCCATCCGG13080.11857782757804787No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13020.11803389258915774No Hit
GGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATTATGTCTTCGGAACTGGGACCAAGGT12910.11703667844285917No Hit
GGAGATGCATTGCCAAAAAAATATGCTTCTTGGTACCAGCAGAAGTCAGG12580.11404503600396347No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12550.11377306850951842No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12480.11313847768914659No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG12470.1130478218576649No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG12470.1130478218576649No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12410.11250388686877477No Hit
GGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGC11910.10797109529469037No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11840.10733650447431857No Hit
AGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCC11630.10543273201320312No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11560.1047981411928313No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT11500.10425420620394116No Hit
ACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG11460.10389158287801442No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11280.10225977791134402No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11070.10035600545022859No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG200.00741152251.6569189
GGTATCA6300.048.6702881
GTATCAA16500.043.0128021
GTGGTAT1550.040.008771
TATCAAC18150.038.896852
ATCAACG19850.035.912623
TCAACGC20100.035.4659424
TGGTATC1750.035.4218862
CAACGCA20200.035.1198845
AACGCAG20250.035.033176
TATATAC501.8811658E-434.4379433
ACGCAGA21600.032.8435977
TCACGTA958.920324E-932.63725725
AATCGTT851.1781776E-732.5642239
CGGATTA552.902503E-431.98576764
ATTATCC1206.91216E-1131.9504653
AGAGTAC21900.031.92337611
CGCAGAG22500.031.5298548
CAGAGTA22700.030.79832510
GATATAT450.00458951230.71174236
Produced by FastQC (version 0.11.5)