FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103073
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC63300.5738514132790848No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40990.371598253243439No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT40780.36969448078232353No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38360.3477557695637551No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGATTACCA36620.3319816548859414No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC35990.32627033750259504No Hit
CCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCT35050.31774868934331635No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29590.2682506053543147No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGATTACCAC29560.26797863785986964No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA29390.266437488724681No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC29380.26634683289319927No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC29160.26435240460060216No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28720.2603635480154079No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28350.2570092822505854No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG27480.24912222491167857No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG27190.2464932057987096No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTT26320.23860614845980277No Hit
GTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAG25610.2321695844246029No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25220.22863400699681705No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG24980.22645826704125654No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24270.22002170300605672No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23240.21068415236344287No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGG22620.2050634908115782No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC22150.20080266673193886No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGATTAC21530.1951820051800742No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA21370.1937315118763672No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20150.18267150043560126No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20000.18131166296337595No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19130.1734246056244691No Hit
GTAGTAGTCAGCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGTGG18990.17215542398372546No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18570.16834787906149457No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA18520.16789459990408614No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC18510.16780394407260446No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATTATGTCTTCGG18470.1674413207466777No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTCTTGGTACCAGCAGAAG18450.1672600090837143No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17850.16182065919481303No Hit
GTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT17670.16018885422814264No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17640.15991688673369758No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTC17500.15864770509295398No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17380.15755983511517369No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17080.15484016017072305No Hit
CTACTACTGTTACTCAACAGACAGCAGTGGTAATCATTATGTCTTCGGAA16960.1537522901929428No Hit
GCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCGGGTGGAGGATGAAGCTGACTACTACTGTTACTCAA16750.15184851773182736No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16550.1500354011021936No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTCTTGGTACCAGCAGAAGTCAGGCCAGGCCC16400.14867556362996828No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16240.14722507032626125No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATT15980.14486801870773738No Hit
CAGTAGTAGTCAGCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGT15910.14423342788736557No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15440.13997260380772622No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15160.13743424052623898No Hit
TCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC15070.1366183380429038No Hit
GACTATCAGTGGGGCCCGGGTGGAGGATGAAGCTGACTACTACTGTTACT15010.13607440305401364No Hit
GTAATACATAGCCGTGTCCTCAGCTCTCAGGCTGTTCGTTTGCAGATACA14440.13090702065955742No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG14260.12927521569288705No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14070.127552754894735No Hit
GCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCC14060.1274620990632533No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13830.12537701493917447No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT13580.12311061915213227No Hit
GTATCAACGCAGAGTACGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTC13500.12238537250027877No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13330.12084422336509006No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12830.11631143179100568No Hit
GGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATTATGTCTTCGGAACTGGGACCAAGGT12620.11440765932989022No Hit
GGAGATGCATTGCCAAAAAAATATGCTTCTTGGTACCAGCAGAAGTCAGG12270.11123470522803114No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG12230.1108720819021044No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12140.1100561794187692No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12050.10924027693543402No Hit
ATGTATTACTGTGCGAGAGAGCCATCTTGTGGTGGTGACTGCCCATCCGG11910.10797109529469037No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11860.10751781613728194No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11760.10661125782246506No Hit
GCTTCATCCTCCACCCGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCC11640.1055233878446848No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11570.10488879702431299No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11500.10425420620394116No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11460.10389158287801442No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11380.1031663362261609No Hit
GGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC11340.10280371290023417No Hit
GGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGC11340.10280371290023417No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11120.10080928460763704No Hit
ACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG11120.10080928460763704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1950.054.638951
TGGTATC2150.047.948972
GTATCAA15250.041.2435951
GGTATCA6200.038.8044551
TATCAAC17200.036.161522
ATCAACG18750.032.9873963
TCCGAGA4600.032.85887570
CAACGCA19300.032.4034235
TCAACGC19350.032.142114
CGTATAT552.932389E-431.92995662
AACGCAG19550.031.8132926
ACGCAGA20700.030.054067
AATGGTA1553.148978E-629.67898270
AGAGTAC21600.028.9727611
CGCAGAG21500.028.944968
GCAGAGT21950.028.6674299
CAGAGTA21850.028.48389410
TCGTATA500.007115097728.07624861
CCTTACA500.00788594427.4907422
GTAGGAT906.7469464E-626.91379239
Produced by FastQC (version 0.11.5)