FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721159
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT16450.228105036476006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4350.049.32642470
CTCGTAT3350.045.5017339
CTTATAC3750.043.7960551
TCGTATG3750.043.45936240
TTATACA3900.042.0969922
CGTATGC3700.041.27577641
GTATCAA30850.040.149341
TATACAC4200.039.904443
TACACAT4650.037.513855
ATACACA4650.037.513854
GGTATCA12300.036.997431
CTCTCTA4600.035.79018829
TCTCGTA4400.035.38350338
TATCAAC36200.034.581752
ACGAGAC4850.033.8652822
GTATGCC4550.033.59800742
GTGGTAT4800.033.5028421
TACATCT4550.033.321134
AACGCAG37700.032.933646
TCTCCGA5100.032.8647611
Produced by FastQC (version 0.11.5)