FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227134
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39210.31952500704894493No Hit
GTGTTAGCCTGCTGACAATAGTAAGTTGCAAAATCTTCGGGCTGCAGGCTGCTGATGGTGAGAATGAAATCTGTC32780.2671264914834077No Hit
GGCTAACACTTTCCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCGCACGAACTGTGGCTGCACCAT26990.21994338026653976No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG26680.21741716878515305No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24800.2020969185109369No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24400.19883729079301854No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22960.18710263100851252No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22360.18221318943163503No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22130.18033890349383197No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21840.17797567339834117No Hit
GTGCAGCCACAGTTCGTGCGATATCCACTTTGGTCCCAGGGCCGAAAGTG21180.1725972876637759No Hit
GGGTATTAGCAGTTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTATGCTGCATC20410.1663225043067831No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19820.16151455342285356No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT18610.1516541795761506No Hit
GCCACAGTTCGTGCGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGG18290.14904647740181595No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18280.14896498670886799No Hit
GTCGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGTTGGTTAGCCTGGTATCAGCAGAAA18140.14782411700759657No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17210.14024548256343644No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17180.14000101048459254No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17120.1395120663269048No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16710.1361709479160385No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14980.1220730580360417No Hit
GCATAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC14450.1177540513097999No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14140.11522783982841318No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14140.11522783982841318No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14080.11473889567072545No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT14060.11457591428482954No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13850.1128646097329224No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13570.11058287033037957No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT13400.10919752855026428No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13330.10862709369962857No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCATTCTCA13230.10781218677014899No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13110.10683429845477349No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12780.10414510558749085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)