FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010869
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGGGTGTCGTCATAGGA31620.3128001748990225No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGG31550.3121077013935534No Hit
CTCCTGCACTGGGAGCAGTTCCAACATCGGGTCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC30000.29677435948673864No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29070.2875743543426497No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23630.23375927048905448No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21380.21150119352754906No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21170.2094237730111419No Hit
GTCCTGGGCCCAGTCTGTGCTGTCACAGCCGCCCTCGGTGTCTGGGGCCCCAGGGCAGAGAATCACCATCTCCTG20990.20764312685422145No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19750.1953764533287696No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19230.19023236443099947No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19040.18835279348758346No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGG18650.18449472681425585No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18270.18073558492742384No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18200.18004311142195478No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16790.16609471652607807No Hit
GTTCCAACATCGGGTCAGGTTATGATGTACACTGGTACCAGCAGCTTCCA16320.16144525156078582No Hit
GTCCTATGACGACACCCTGAGTGGTTCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG15730.1556086891575466No Hit
ATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG15460.15293771992216598No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA15440.15273987034917483No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT15400.1523441712031925No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15350.15184954727071462No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC15300.15135492333823672No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGGG15220.15056352504627207No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15180.15016782590028974No Hit
GGCCATCACTGGCCTCCAACCTGAGGATGAGGCTGATTATTACTGCCAGT15030.14868395410285604No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14520.1436387899915815No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14020.13869255066680253No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13930.13780222758834232No Hit
GTGCTGTCACAGCCGCCCTCGGTGTCTGGGGCCCCAGGGCAGAGAATCAC13710.13562588228543956No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13550.13404308570151027No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13320.13176781561211195No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13020.12880007201724455No Hit
GTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTA13020.12880007201724455No Hit
TCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGGTTGGAGGCCAGT12990.12850329765775784No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12550.12415060705195233No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG12340.12207318653554516No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12330.12197426174904959No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12250.12118286345708495No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11940.11811619507572198No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11890.11762157114324408No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11850.11722587199726175No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11500.11376350446991648No Hit
GTATATTACTGTGCGAGGGCGAGCAGTTGTAGTGGTGGTAGCTGCTACTCATCAAACTGGTTCGACCCCTGGGGC11370.11247748224547394No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11360.11237855745897837No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11330.11208178309949161No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGTCACAGCCGCCCTCGGTGTCTGGGGCCCCAGGGCAGAGAATCAC11270.11148823438051816No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11260.11138930959402257No Hit
TGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC11060.10941081386411097No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11060.10941081386411097No Hit
ACTCAGGGTGTCGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAG11060.10941081386411097No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10890.10772909249368613No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA10650.10535489761779222No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10590.10476134889881873No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10470.10357425146087179No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGTCACAGCCGCCCTCGGTGTCT10350.10238715402292482No Hit
GATTATTACTGCCAGTCCTATGACGACACCCTGAGTGGTTCGGTGTTCGG10180.10070543265249998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)