FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010869
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGGGTGTCGTCATAGGA30800.304688342406385No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29000.2868818808371807No Hit
CTCCTGCACTGGGAGCAGTTCCAACATCGGGTCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC28200.2789678979175343No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGG27250.26957004320045425No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21560.2132818396844695No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20890.20665387898926565No Hit
GTCCTGGGCCCAGTCTGTGCTGTCACAGCCGCCCTCGGTGTCTGGGGCCCCAGGGCAGAGAATCACCATCTCCTG20370.20150979009149556No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19820.19606892683423868No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19420.19211193537441548No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19170.18963881571202598No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18380.1818237575788752No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGG18280.1808345097139194No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17510.17321730115375977No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17500.1731183763672642No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17190.17005170798590125No Hit
GTCCTATGACGACACCCTGAGTGGTTCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16300.16124740198779466No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15830.1565979370225024No Hit
ATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG15650.154817290865582No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC15180.15016782590028974No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT14850.14690330794593562No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA14840.14680438315944003No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14720.1456172857214931No Hit
GTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTA14550.14393556435106825No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14300.14146244468867875No Hit
GTTCCAACATCGGGTCAGGTTATGATGTACACTGGTACCAGCAGCTTCCA14270.14116567032919203No Hit
GGCCATCACTGGCCTCCAACCTGAGGATGAGGCTGATTATTACTGCCAGT14060.13908824981278484No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGGG13940.13790115237483788No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13600.13453770963398817No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13580.13433986006099702No Hit
GTGCTGTCACAGCCGCCCTCGGTGTCTGGGGCCCCAGGGCAGAGAATCAC13260.13117426689313846No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12970.12830544808476665No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12820.12682157628733298No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12610.1247441557709258No Hit
TCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGGTTGGAGGCCAGT12590.12454630619793464No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12580.12444738141143907No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12380.12246888568152747No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12360.12227103610853632No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12100.11969899165965125No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11520.11396135404290764No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11510.11386242925641205No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG11490.1136645796834209No Hit
TGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC11480.11356565489692531No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11370.11247748224547394No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGTCACAGCCGCCCTCGGTGTCTGGGGCCCCAGGGCAGAGAATCAC11240.1111914600210314No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11230.11109253523453583No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11080.10960866343710214No Hit
GATTATTACTGCCAGTCCTATGACGACACCCTGAGTGGTTCGGTGTTCGG11060.10941081386411097No Hit
GTATATTACTGTGCGAGGGCGAGCAGTTGTAGTGGTGGTAGCTGCTACTCATCAAACTGGTTCGACCCCTGGGGC10930.10812479163966844No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10750.10634414548274802No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10520.10406887539334969No Hit
GGGTGTCGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGGTTGG10390.10278285316890715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTTA200.006951811752.49988652
GTATCAA16800.040.6089321
TATCAAC18050.037.5918662
GGTATCA6750.037.584241
TGGTATC2650.033.622762
ATCAACG20200.033.590753
TCAACGC20700.032.779384
AACGCAG20750.032.702016
CAACGCA20800.032.6217885
GTGGTAT3000.031.997391
ACGGGTA553.375622E-431.1724616
AGAGTAC22300.030.60084711
GTCTAGG450.00476737230.4737031
CGCAGAG22750.029.9881348
CAGAGTA22950.029.7341610
GCAGAGT24100.028.5998829
CTAAAAT950.0084319227.11359470
GTACTTT6650.024.7516914
AACCGTA700.001384894124.4841657
TAATACC700.001386891424.478114
Produced by FastQC (version 0.11.5)