FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715532
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT12120.16938445799768564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3750.055.32390270
TCGTATG2450.049.5030240
CTCGTAT2250.047.6990739
CTTATAC3250.043.235061
CGTATGC3100.042.5248941
TATACAC3600.040.909893
GTATCAA31950.040.010461
TTATACA3700.037.9528582
TACGGAC655.7665056E-736.88476615
ACGAGAC3900.036.02669522
GGTATCA13650.035.4015271
TATCAAC36350.035.2394832
TATGCCG3850.035.20169443
CGAGACA3700.035.1979623
GCCGTCT3950.034.3909846
GTGGTAT5600.034.2716941
CACGAGA4000.034.26450721
AACGCAG38350.033.491016
TCTCGTA3000.033.44738438
ATCAACG38450.033.4039083
Produced by FastQC (version 0.11.5)