FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715532
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25230.35260477518825156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33350.038.6852381
TATCAAC37350.034.245822
GGTATCA14700.032.591961
AACGCAG39200.032.4551356
CAACGCA39950.032.0170565
ATCAACG40300.031.8238543
TCAACGC40650.031.4657144
ACGCAGA41400.030.6649997
CGCAGAG41700.030.4550368
AGAGTAC40400.030.24879611
CAGAGTA41200.029.66144210
GCAGAGT42650.029.5358899
GTGGTAT6750.028.8982031
CTTAGAC756.537752E-527.3600273
TGGTATC7200.026.6000272
GAGTACG40900.026.11274712
AGTACGG40550.025.91604813
CCTATCC1209.2595656E-825.6500263
TACGGGG18200.023.69681715
GTACGGT750.002085002622.82075514
Produced by FastQC (version 0.11.5)