FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171619
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38920.3321899013245774No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCCAGCACTCAGGCTGCTATCCCATGTT33530.2861851847742312No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27680.2362542772010355No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26000.22191514476975877No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25190.2150016344903932No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22000.18777435326671896No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT21330.1820557706899598No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18780.16029101610677193No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18270.15593806519013434No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18150.15491384144504314No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17910.15286539395486076No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17770.15167046625225436No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC17490.14928061084704158No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17130.14620793961176798No Hit
CCCCAGCACTCAGGCTGCTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC17060.1456104757604648No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16580.1415135807801No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16350.13955048526867522No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16110.13750203777849285No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15870.13545359028831044No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15430.1316981032229761No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15130.1291375438602481No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14920.1273451523063385No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14540.12410177711354971No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG14510.12384572117727691No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14340.12239473753839772No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14070.12009023411194253No Hit
GTGCTGGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCC13800.11778573068548735No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAA13690.11684685891915375No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13370.11411559559891056No Hit
GTGATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCA13100.11181109217245537No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12830.10950658874600018No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC12770.1089944768734546No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCCAGCACTCAGG12640.1078849011496058No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12330.10523898980812021No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAGTGATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG12310.10506828585060501No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCCAGCACTCAGGC12290.10489758189308981No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12100.10327589429669543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG6750.050.10661770
GGTATCA11850.041.843261
GTATCAA29250.040.3181
GTGGTAT3200.039.784671
CTTATAC6050.037.1023671
TATCAAC32800.036.3396072
TCGTATG4500.035.2778440
CGATTGT951.7373062E-434.9039970
CTCGTAT4500.034.40649839
ATCAACG34750.034.3004043
TCAACGC35200.033.8619084
CAACGCA35400.033.6705975
TCTCGTA4600.033.55698838
TATCTCG4800.033.40984336
TTATACA6450.033.221892
TATACAC6600.032.974143
AACGCAG36750.032.7070356
ACGAGAC6200.032.426322
TACACAT6350.032.163275
CCCACGA6550.031.70859119
Produced by FastQC (version 0.11.5)