FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159971
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCCCCATGTAGGCTGTGTCTGTAGCCGAGTCCTCGGT45900.3956995476611053No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36810.31733551959488643No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT36490.3145768299379898No Hit
GTCTATTACTGTGCATCGGGAATCCCCTATCACGATATTTTGACTGGTCATTGGTACTACTTTGACGACTGGGGC33350.287507187679692No Hit
GTCTGGGGCTGAGGTGGAGAAGCCCGGGGCCTCGGTGAACATCTCCTGCG32670.2816449721587867No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC31930.27526550232721336No Hit
TCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGACTTGAGTGGATGGG29420.25362703033093065No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28740.2477648148100254No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27730.23905770058044556No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25450.21940203677505732No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24950.21509158418615637No Hit
ACCTGGTACAGTCTGGGGCTGAGGTGGAGAAGCCCGGGGCCTCGGTGAACATCTCCTGCGAGGTTTCCGGATACA24730.21319498504703996No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23820.20534996133524025No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22740.19603938374321428No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT22520.19414278460409784No Hit
ATCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGACTTGAGTGGATGG22100.19052200442942108No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA20920.1803493363196149No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC20880.18000450011250282No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT20740.17879757338761054No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGCATTGAACACTC20460.17638371993782603No Hit
ACATGGGACACCAGCCTGAGTGTTCAATGCGTGTTCGGCGGAGGGACCAA20410.17595267467893594No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19760.17034908631336473No Hit
GTGTATGGATAAATCAGTGAGGGTGTATCCGGAAACCTCGCAGGAGATGTTCACCGAGGCCCCGGGCTTCTCCAC19410.16733176950113407No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19400.16724556044935607No Hit
GCCCCATGTAGGCTGTGTCTGTAGCCGAGTCCTCGGTCATGGTGACTCTG18990.1637109893264573No Hit
ACATGGGGCTGAGCAGCCTGACATCTGAGGACACGGCCGTCTATTACTGT18780.16190059923911893No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT18690.16112471777311674No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18680.16103850872133874No Hit
CATGTAGGCTGTGTCTGTAGCCGAGTCCTCGGTCATGGTGACTCTGCCCT18450.1590557005304443No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA18370.15836602811622016No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18230.15715910139132788No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18020.1553487113039895No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17790.15336590311309506No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG17730.15284864880242696No Hit
GCCTACATGGGGCTGAGCAGCCTGACATCTGAGGACACGGCCGTCTATTA17290.14905545052419414No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA17270.1488830324206381No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA17170.14802094190285792No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16640.14345186215862293No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGG16290.1404345453463923No Hit
GCTCAGCCCCATGTAGGCTGTGTCTGTAGCCGAGTCCTCGGTCATGGTGA15810.1362965108610474No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG15650.1349171660325991No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG15500.1336240302559288No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGCA15410.13284814878992665No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC15390.13267573068637062No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC15260.13155501301325637No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15140.13052050439192014No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15110.13026187723658608No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15060.129830831977696No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG14950.12888253240813777No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC14920.12862390525280373No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14780.12741697852791145No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14520.125175543181683No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14410.12422724361212478No Hit
GTCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG14380.12396861645679073No Hit
CAGTAATAGACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCCCCATGTA14310.12336515309434459No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT14200.12241685352478639No Hit
GTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAA13690.11802019188410745No Hit
GTCATGGTGACTCTGCCCTGAAATTTCGGTGCGTAGATTGTTTTACCATCTTCAGGATCAAAAGTTCCCATCCA13670.11784777378055142No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13620.11741672852166132No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC13330.11491666602009877No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC13200.11379594834698453No Hit
ACCCTGGACATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTG13080.11276143972564832No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13040.11241660351853625No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG12960.11172693110431209No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCATCTCCTC12850.11077863153475388No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12800.1103475862758638No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA12740.10983033196519568No Hit
CCCCTACAGAAGCCCCCAGAGCGCAGCACCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAG12710.10957170480986161No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12650.10905445049919352No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12530.1080199418778573No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACC12500.10776131472252323No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12440.10724406041185512No Hit
CAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGC12360.10655438799763096No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA12350.10646817894585296No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA12330.10629576084229692No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC12090.10422674359962447No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12000.10345086213362231No Hit
GGGTTCCCTGGCCCCAGTCGTCAAAGTAGTACCAATGACCAGTCAAAATA11980.10327844403006627No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11980.10327844403006627No Hit
ACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCTACAGGCACCCACGCCCAGATCCACCTGGTA11910.10267498066762012No Hit
CACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCTACAG11890.1025025625640641No Hit
CCCCAGGAGTTCAGGTGCTGGGCACGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTCTCTTGTC11720.10103700868383779No Hit
CTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAT11720.10103700868383779No Hit
GGGTGCGACAGGCTCCTGGAAAAGGACTTGAGTGGATGGGAACTTTTGATCCTGAAGATGGTAAAACAATCTACG11640.10034733626961363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGGTA350.00599524954.49973370
AATGGTA2050.046.52416270
TATACAC1650.041.6961823
GTATCAA15400.041.107611
AGGGGGG2200.040.46192670
GTATTGG350.001366161239.3203241
GTAATGA350.001366161239.3203241
GTGGTAT2050.038.6010441
TATCAAC16450.038.477082
TTATACA1800.038.22152
CTTATAC1850.037.1948971
TCGTATG1500.036.94425240
CTTAGCC400.002633809634.399353
AACGCAG19200.033.3243686
ATCAACG19050.033.2256163
TCAACGC19150.033.0521164
AGACGGA1800.032.50649325
CAACGCA19700.032.303965
CACTGAT12850.031.6678170
AGAGTAC19250.031.4521911
Produced by FastQC (version 0.11.5)