FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159971
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCCCCATGTAGGCTGTGTCTGTAGCCGAGTCCTCGGT43650.37630251101105117No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT39170.3376808558144988No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37520.32345636227112573No Hit
GTCTATTACTGTGCATCGGGAATCCCCTATCACGATATTTTGACTGGTCATTGGTACTACTTTGACGACTGGGGC32460.2798345820714483No Hit
GTCTGGGGCTGAGGTGGAGAAGCCCGGGGCCTCGGTGAACATCTCCTGCG32400.2793173277607802No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC31730.273541321291653No Hit
TCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGACTTGAGTGGATGGG27890.24043704540889385No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26030.22440216177818237No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25160.21690197427349478No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23970.20664309711191053No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23000.19828081908944273No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21870.18853919623852664No Hit
ATCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGACTTGAGTGGATGG21830.18819436003141457No Hit
ACCTGGTACAGTCTGGGGCTGAGGTGGAGAAGCCCGGGGCCTCGGTGAACATCTCCTGCGAGGTTTCCGGATACA21460.18500462511562787No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20790.17922861864650066No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20790.17922861864650066No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT20580.17741822855916226No Hit
ACATGGGACACCAGCCTGAGTGTTCAATGCGTGTTCGGCGGAGGGACCAA20080.17310777597026134No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC20030.17267673071137124No Hit
GTGTATGGATAAATCAGTGAGGGTGTATCCGGAAACCTCGCAGGAGATGTTCACCGAGGCCCCGGGCTTCTCCAC19920.17172843114181302No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19890.17146980398647899No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT19480.1679352328635802No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC19130.16491791605134956No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT18930.16319373501578918No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18910.16302131691223315No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18720.1613833449284508No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA18620.1605212544106706No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18520.15965916389289042No Hit
CATGTAGGCTGTGTCTGTAGCCGAGTCCTCGGTCATGGTGACTCTGCCCT18470.15922811863400033No Hit
GCCCCATGTAGGCTGTGTCTGTAGCCGAGTCCTCGGTCATGGTGACTCTG18250.15733151949488391No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17330.14940028673130623No Hit
ACATGGGGCTGAGCAGCCTGACATCTGAGGACACGGCCGTCTATTACTGT17130.14767610569574582No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT17060.1470726423332997No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT16740.14431395267640312No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16670.143710489313957No Hit
GCCTACATGGGGCTGAGCAGCCTGACATCTGAGGACACGGCCGTCTATTA16620.1432794440550669No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16380.14121042681239446No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA16360.14103800870883842No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16140.139141409569722No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGGCAGAAGGTCACCATCTCCTG16090.13871036431083192No Hit
GTCATGGTGACTCTGCCCTGAAATTTCGGTGCGTAGATTGTTTTACCATCTTCAGGATCAAAAGTTCCCATCCA16030.1381931100001638No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG16030.1381931100001638No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA15550.1340550755148189No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15440.13310677594526069No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA15200.13103775870258824No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG15130.13043429534014211No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGGCAGAAGGTCAC14890.12836527809746967No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC14690.12664109706190932No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGCATTGAACACTC14680.1265548880101313No Hit
GGGTGCGACAGGCTCCTGGAAAAGGACTTGAGTGGATGGGAACTTTTGATCCTGAAGATGGTAAAACAATCTACG14360.1237961983532347No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14280.12310652593901054No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14250.1228478987836765No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG14150.1219858082658963No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14150.1219858082658963No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14050.12112371774811613No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13950.12026162723033593No Hit
CAGTAATAGACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCCCCATGTA13860.11948574576433377No Hit
ACCCTGGACATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTG13680.11793398283232942No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG13370.11526150222721085No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC13370.11526150222721085No Hit
GCTCAGCCCCATGTAGGCTGTGTCTGTAGCCGAGTCCTCGGTCATGGTGA13250.11422699360587464No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA12640.10896824144741549No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12580.10845098713674739No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTCTCTTGTCC12560.10827856903319136No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC12370.106640597049409No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA12230.10543367032451673No Hit
GGGTTCCCTGGCCCCAGTCGTCAAAGTAGTACCAATGACCAGTCAAAATA12210.10526125222096071No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC12050.1038819073925124No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC11780.10155426299450589No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGG11760.10138184489094987No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG11740.10120942678739381No Hit
GTCTGTAGCCGAGTCCTCGGTCATGGTGACTCTGCCCTGAAATTTCGGTG11700.10086459058028176No Hit
CTATTACTGTGCATCGGGAATCCCCTATCACGATATTTTGACTGGTCATT11660.10051975437316968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1850.046.4446451
GTATCAA16000.040.8132361
TATACCG350.001375587439.2653585
TCCTATG451.0260756E-438.1762962
TATCAAC17250.037.844332
TCCTAGA400.002649135734.358672
CTTATAG400.002649135734.358672
ATCAACG19950.032.8947643
AACGCAG20000.032.6393286
CAGTAGG800.00355800232.34913670
TCAACGC20250.031.8970434
CACTGAT15200.031.4978470
CAACGCA20650.031.445565
AGAGTAC20700.030.55289311
CTACGGT450.004717463630.5397194
ATACCGA450.004717463630.5397196
ACTCAAG6800.030.44624370
TATAAGA1700.030.3164712
CGCAGAG21600.029.9137948
TTTAAGT900.006329871228.75478770
Produced by FastQC (version 0.11.5)