FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796979
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5650.042.6004141
GTATCAA12900.037.3166431
CTCGTAT905.013135E-934.61932839
TATCAAC14600.032.717562
TCGTATG1052.2369932E-829.69435340
TCAACGC16050.029.5476514
CAACGCA16300.029.0944675
ATCAACG17000.027.8964613
AACGCAG17200.027.5720836
CGCAGAG18950.025.0258488
CGTATGC1251.2056444E-724.96062341
AGAGTAC19050.024.89447811
ACGCAGA19350.024.5085187
CAGAGTA19650.024.48411610
CTAGCGC1151.7296297E-623.94679528
ATCTAGA902.1743316E-422.9230791
GCAGAGT21000.022.582859
TGGTATC2450.022.4425832
ACTAGCG1403.8542203E-722.12940227
TATACTA1103.3654444E-521.8687675
Produced by FastQC (version 0.11.5)