FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167815
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAGTGGCCCATGTCTGACAGTAATAGT35600.3048428047250635No Hit
GTCCCAGAGCTGGAGCCTGAGAAGCGATGAGGGATCCCGTCCCCCAGGGT33250.2847197544131562No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAGTGGCCCATGTCTGA32770.2806095143494475No Hit
CTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT32130.27512919426450255No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28910.24755633383712317No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24380.20876594323587214No Hit
CTCCTGTACAGCCTCTGGATTCATCTTTGGTTCCTATGCCATGAACTGGGCCCGCCAGGCTCCAGTAAAGGGG23120.19797656306863673No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21870.1872728129027286No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21790.18658777289211048No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21040.18016552279256562No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21000.17982300278725655No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT20860.17862418276867484No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20580.17622654273151142No Hit
GCTCTGGGACTGAGCGCTACCTCACCATCTCCAGGCTCCAGTCTGAGGAT20270.17357201269036618No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC18590.15918617246738567No Hit
CACCTGCACTCTGGCCAGTGGGCACAGCACCTACGCCATCGCGTGGCTTC18120.15516156240500423No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17870.15302081237182258No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC17710.15165073235058635No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17590.15062317233465916No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17580.15053754233333191No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16880.14454344224042334No Hit
GGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC16830.14411529223378705No Hit
GGGCTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16140.13820682214220575No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16030.13726489212760581No Hit
CCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT15650.13401095207716976No Hit
CTCTGGGACTGAGCGCTACCTCACCATCTCCAGGCTCCAGTCTGAGGATG15600.13358280207053344No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14220.12176586188737085No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13730.11756999182233488No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCTGGAGATGGTGAGGTAGC13670.11705621181437129No Hit
CCTGCACTCTGGCCAGTGGGCACAGCACCTACGCCATCGCGTGGCTTCAG13360.11440168177322607No Hit
GGGTGTGGCTGCCGTCACTTTTAACTTTCATTAAGTATCGAGGGCCCTTC13330.11414479176924427No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT13240.11337412175729888No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13060.11183278173340812No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13020.11149026172809905No Hit
GCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG12990.11123337172411725No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12990.11123337172411725No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12980.11114774172279No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12920.11063396171482641No Hit
GACTATTACTGTCAGACATGGGCCACTGGCATTGATTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT12810.10969203170022648No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12670.10849321168164479No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12560.10755128166704488No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12530.10729439166306308No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAG12460.10669498165377223No Hit
GCCACACCCTGGGGGACGGGATCCCTCATCGCTTCTCAGGCTCCAGCTCTGGGACTGAGCGCTACCTCACCATCT12370.10592431164182683No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12320.10549616163519052No Hit
TCCTCAGACTGGAGCCTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGCTGGAGCCTGAGAAGCGATGAGGGATC12230.10472549162324513No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12220.10463986162191785No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12050.10318415159935436No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11760.10070088156086367No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACATGGGCCACTGGCATTG11750.10061525155953639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3550.044.6421431
GTATCAA16600.039.225571
TGGTATC4050.039.112342
GGTATCA7400.034.917661
TATCAAC19000.034.073382
ACGCGAT8200.033.00845770
CAACGCA20850.031.0500835
TCAACGC20900.030.9757984
ATCAACG21150.030.6096553
AACGCAG21550.030.0427846
ACTTTCA14500.029.13849670
GTGTCCA10450.028.42833370
GTATAAG2000.027.561671
AGAGTAC23400.027.3873411
CGCAGAG23750.027.2680458
CAGAGTA23850.026.870610
GCAGAGT24850.026.2041199
TATTAGA851.4616568E-424.3076692
GTGTATA1452.0449079E-823.7600611
GGGTTTA4650.022.968061
Produced by FastQC (version 0.11.5)