FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271637
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT17410.6409288867127821No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT3880.14283768411519787TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT2760.10160618766957372RNA PCR Primer, Index 11 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2500.080.0586170
TGACTAT200.005786555454.98024766
TATACAC2500.052.6417433
TTATACA2550.051.609552
CTCTTAT200.00799045550.6730421
AAGGGGG2550.050.2811569
CTTGAAA2800.048.68275557
TTGAAAA2900.047.059358
GTGGTAT606.724804E-945.04271
AAAAAGG2650.044.2608366
TACACAT3000.043.8681145
ATACACA3050.043.1489684
GGAGCTA3100.042.77594429
CTTATAC3100.042.499971
TACATCT3150.042.41963234
AAAGGGG2800.042.0699468
CTCGTAT3300.042.0485439
TGAAAAA3350.041.90850459
CGTATGC3300.041.1609441
ACACATC3300.040.9026766
Produced by FastQC (version 0.11.5)