FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271637
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34171.2579287799526575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACA150.002559376967.55672
CGGCTAA200.00790121150.8172124
GCTAATC200.00797646450.69552616
GTGTATA851.8189894E-1243.7534331
GTGGTAT1000.040.5713651
GTATCAA6450.038.789161
GGTAATA350.001493370338.603832
TGTATAA1001.0913936E-1137.1561852
CTTCTGA400.002098415336.0196360
ACTGTTA750.00223567535.54599870
GTAATAA400.00287600233.778353
GCTAAGA400.00287600233.778352
TGGTATC1103.6379788E-1133.7783472
TATCAAC7700.032.9009932
CAGAGTA7700.031.60822510
AGAGTAC7750.031.404311
AACGCAG8350.030.7443686
ACGCAGA8500.030.207387
GGGTAAT450.00509635330.0528641
TAATGAC450.005114752330.0307277
Produced by FastQC (version 0.11.5)