FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716179
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29080.40604374046153263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35450.036.6555561
GGTATCA15900.035.0560461
TATCAAC39750.032.758022
GTGGTAT6350.031.2339361
AACGCAG42000.030.9217536
TCGATAG450.004737753430.51133729
CAACGCA43050.030.2469525
TCAACGC43300.030.1512454
ATCAACG43550.029.8996853
TGGTATC6750.029.8729462
ACGCAGA44150.029.3405747
AGAGTAC43300.029.13532611
CAGAGTA43600.029.0132710
CGCAGAG45100.028.5729628
GCAGAGT46600.027.5122939
AGTACGG46150.023.63199613
GAGTACG47050.023.47060612
GTACCTA1103.495626E-521.7609461
TACGGGG19900.021.47975715
GTACGGG48850.020.71612714
Produced by FastQC (version 0.11.5)