FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048136
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCACTCAGG20800.19844752970988497No Hit
GTGATAATCAGCTTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG20700.19749345504781823No Hit
GATTATCACTGTGCAGCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGG20150.19224604440645107No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC18320.17478647809062944No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16550.1578993565720479No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCACTCAGGCTGTCATCCCATG15860.1513162414037873No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14840.14158467985070639No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14120.13471534228382576No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC14020.13376126762175902No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT13970.1332842302907256No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13900.1326163780272789No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13120.12517459566315822No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12820.12231237167695795No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC12710.12126288954868451No Hit
GTGATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGTGAGAACTGGTTCTTGGACTTGTCTACGGA12350.1178282207652442No Hit
GTCTCTGGTGGCTCCATCAGCAGTCCTAGTTACTACTGGACCTGGATTCG12320.11754199836662418No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12080.11525221917766397No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11830.1128670325224971No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACC11560.11029103093491684No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC11300.10781043681354328No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11200.10685636215147652No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11040.10532984269216973No Hit
ATGCTGCACAGTGATAATCAGCTTCATCCTCGGACCGGAGCCCACTGATG10950.10447117549630965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9250.041.742511
GTATCAA22550.041.5838431
TATCAAC25700.036.4626242
GTGGTAT3000.034.4748421
TCAACGC28100.033.103174
CAACGCA28350.032.8112565
AACGCAG28650.032.347436
TGGTATC3250.031.8016782
TCGCTAT450.004647702430.63260724
AGAGTAC30800.029.865711
CGCAGAG31250.029.6561268
CAGAGTA31350.029.5615310
ACGCAGA31450.029.4675337
GCAGAGT32550.028.3658589
TAATATG658.727516E-426.5013984
ATCAACG36650.025.5686053
GTGTAAG951.05661275E-525.4025171
AGTACGG25750.024.48420513
GAGTACG26450.023.83622712
CTACGGA5350.023.0405269
Produced by FastQC (version 0.11.5)