FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048136
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC21030.20064190143263852No Hit
GATTATCACTGTGCAGCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGG18800.1793660364685499No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT15850.15122083393758062No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15670.14950349954586045No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCACTCAGG15570.1485494248837937No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15550.14835860995138034No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14920.1423479395803598No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14840.14158467985070639No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13000.12402970606867811No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC12450.11878229542731096No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12430.11859148049489761No Hit
GTGATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGTGAGAACTGGTTCTTGGACTTGTCTACGGA12240.11677873863697077No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCACTCAGGCTGTCATCCCATG12020.11467977438042391No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11940.11391651465077052No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC11780.1123899951914637No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11740.11200836532663701No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11520.10990940107009015No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACC11070.10561606509078975No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10900.10399413816527625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10740.10246761870596946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23950.042.5121421
GGTATCA9800.039.311421
TATCAAC27550.036.4367262
TCAACGC29400.034.027014
TGGTATC3350.033.86472
AACGCAG29950.033.4021386
CAACGCA30150.033.294595
GTGGTAT3550.032.944081
TAACCGT1208.0035534E-1131.5190017
CGCAGAG33000.030.4380258
ACGCAGA33200.030.2416697
AGAGTAC34300.029.68838711
CAGAGTA35000.028.89803510
GTGTATA2050.028.524751
GCAGAGT35800.028.2522689
CTAACCG1254.311005E-927.5022416
AGTACGG31150.024.51910813
AGTACTT10500.024.24667213
ATCAACG41600.024.1305733
GAGTACG31950.023.79749112
Produced by FastQC (version 0.11.5)