FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173871
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACGTGGGATGACAACCTGGGTGGCCCCAGGTGGGT28100.23937894368290893No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24560.20922230807303357No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC21010.17898048422697213No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCACCTGGGGCCA19840.16901346059319974No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCACCTGGGGCCACCCAGGTTGTCATCC19640.16730969586947797No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT18170.154787025150123No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG18110.15427589573300643No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17810.15172024864742378No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17100.14567188387821148No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16770.14286067208407058No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16240.13834569556620788No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15570.13263808374173994No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14660.12488595424880587No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG14370.1224154953994093No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13880.11824127182629098No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13830.11781533064536052No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13820.11773014240917443No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13610.11594118944926657No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13280.11312997765512565No Hit
CTATAGGAGTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC13170.11219290705707867No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC13010.11082989527810125No Hit
CTCCTATAGATGAGGACCTTGGGGGCCATTCCTGGGAACTGCTGGTACCA12820.10921131879056557No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12410.10571860110693596No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12280.10461115403651679No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTCAAGATATA12200.10392964814702808No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12180.1037592716746559No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12040.10256663636805066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5150.041.2975581
GGTATCA13850.036.904251
TGGTATC5950.036.315292
GTATCAA29650.036.2125851
TATCAAC33700.031.6516532
TCCGAAA450.003997013431.59173266
ATCAACG35900.029.7119963
TCAACGC36000.029.6294634
AACGCAG36250.029.5197376
CAACGCA36350.029.3441755
GCCGTTA500.0079369427.45459215
AGAGTAC39750.027.1092511
CGCAGAG39900.026.9167358
CAGAGTA40150.026.75369510
CCGTTAA658.9283695E-426.39864316
GCAGAGT42150.025.6459879
ACGGGTA951.094734E-525.28712316
ATATTGG12450.025.00634270
GTATTAA700.001378973224.5025831
AGTACGG39650.023.97516313
Produced by FastQC (version 0.11.5)