FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417065
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT17730.4251135914066153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3250.087.09350670
CTATCCT150.002462301468.225274
TATCGTA200.007238788551.960138
TTACACA200.007691567851.1689534
TACACAA200.007691567851.1689535
CTTATAC3400.050.1836931
TTATACA3450.049.4386062
TATACAC3550.048.0459673
AAAGGGG3750.046.3558268
CTTGAAA3850.046.0064857
ATACACA3750.045.4835134
TTGAAAA4000.045.20625758
AAAAGGG3750.044.22551367
TACACAT3900.043.7341465
AAAAAGG3850.042.8217866
TCGTATG4150.041.8573240
AAGGGGG4500.041.72696769
CTCGTAT3850.040.532839
GTCTTCT4550.040.14086549
AGACGTA4350.040.0806225
Produced by FastQC (version 0.11.5)