FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417065
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35470.8504669535923657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTAA200.007447912451.58725429
ATATGCT200.00761257151.302753
CTACACT200.00761257151.302753
TACACTC200.00761257151.302754
TATACAC306.582401E-445.6024483
GTATCAA23550.036.765411
GGTATCA10200.033.8868331
TATCAAC26050.033.2171362
AACGCAG27100.031.4253036
CAGAGTA27200.030.1961910
AGAGTAC27000.030.16636711
GCAGAGT27850.030.1058379
ACGCAGA29350.029.0266467
CAACGCA29800.028.6928145
CGCAGAG29400.028.6350258
ATCAACG30650.028.008683
TCAACGC30850.027.7162344
CAGGTAT1005.3709846E-727.377881
AGGGACC500.00806374427.3614672
AGGTATC907.3730644E-626.6014272
Produced by FastQC (version 0.11.5)