FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115530
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28070.2516292703916524No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23050.20662823949154216No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18770.16826082669224493No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18410.16503366112968723No Hit
AGTTTACACTGGTACCAACAGAAACCAGGTCAGTCTCCAAAGCTCCTCAT18360.1645854436904431No Hit
ACCTATTACTGTCTTCAGAGTAGTAGTTTACCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA17990.16126863464003657No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17820.15974469534660654No Hit
GTAATATACAGCCGTGTCCGCGGCGGTCATAGAGTTCACCTTCAGGGAGA17430.15624859932050236No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17290.1549935904906188No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16050.14387779799736447No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16010.14351922404596917No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15790.14154706731329503No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15650.14029205848341147No Hit
GTAATAGGTTGCAGCATCTTCAGCTTCCAGTCTATTGATGGTGAGGGTGA15180.13607881455451667No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14940.13392737084614487No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14330.12845911808736654No Hit
GTGTAAACTAGTAGCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTT14290.12810054413597124No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14250.12774197018457595No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13650.12236336091364644No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13610.12200478696225113No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13140.11779154303335633No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13130.11770189954550751No Hit
GTATATTACTGTGCGAGAGGCGAGACCTGGATAGCACCTCGTTCGTACTACTTTGACTACTGGGGCCAGGGAAAC12920.11581938630068218No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12220.10954434215126442No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11830.10604824612516024No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11600.10398644590463726No Hit
GAGTAGTAGTTTACCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11460.10273143707475371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2800.041.6308331
GTATCAA20050.038.8155481
TAACGGC459.9980774E-538.3446236
GGTATCA9300.036.8647461
TATCAAC22400.034.4233782
ATCAACG23650.033.038683
TCAACGC23850.032.7601554
CAACGCA24250.032.5024155
TGGTATC3800.031.5648272
AACGCAG25350.031.49766
AGAGTAC26300.030.12355811
CGCAGAG26550.029.8345578
TACTGAA9500.029.48391570
TTAACGG605.427058E-428.74548735
TTTAACG605.438532E-428.73511734
GTATTAA605.624845E-428.5701791
GCAGAGT28250.028.2870399
ACGCAGA28700.027.8260387
CGTCTAA1404.110916E-1027.41458950
AATATAC6900.027.3169733
Produced by FastQC (version 0.11.5)