FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119326
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAGGTCTCAGACTCTTCATTTGCAGATACA42370.37853136619715794No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGAA38700.34574377795208905No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37910.33868595922903605No Hit
GTCTATTACTGTGCGACTCTTTCCGGGGTGGCAGTTATTTTCCCCTACCAGTACGACGGTTTGGACGTCTGGGGC37820.33788190393147305No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC37280.33305757214609505No Hit
GTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT30770.2748975722890382No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC27590.2464876184418123No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26540.23710697330357736No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25390.22683293339027236No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24870.22218728055990838No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24320.21727360929702338No Hit
GGTCTAGTCAGAGCCTCGAATACAGCGACGGAAACATCCACTTGAATTGGTTTCACCAGAGGCCAGGCCAGTCTC24170.21593351713441838No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24080.2151294618368554No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23980.2142360670617854No Hit
GGTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT23810.21271729594416638No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23460.2095904142314214No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23420.2092330563213934No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21800.19476006096525944No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG21610.19306261089262644No Hit
ACTCTACACTGGGTCCGCCAGGCCCCAGGCAAGGGACTGGAGTGGGTGGC21220.18957837126985347No Hit
AGGTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT21000.18761290276469947No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20010.1787682944915065No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTCAA19780.1767134865088455No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAAGTAGGCGCCTTGGAGACTGGCCTGGCCTCTGGTGAAA19000.16974500726329952No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18810.16804755719066652No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18750.16751152032562452No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18170.16232983063021855No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17670.15786285675486855No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17450.15589738824971455No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17450.15589738824971455No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17390.15536135138467258No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16780.14991164325674558No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16770.14982230377923858No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16730.14946494586921058No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGAATA16720.14937560639170358No Hit
CTCCAGTCCCTTGCCTGGGGCCTGGCGGACCCAGTGTAGAGTATAGCCAC16170.14446193512881858No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTACTTTATAAGGTTTCTAACCGGGAC16090.14374721930876258No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15970.14267514557867858No Hit
GTGTAGAGTATAGCCACTAAAGGGGAATCCAGAACCTGCACAGGAGAGTC15860.1416924113261016No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTGAGTGTGAAATCAGTGCCTGACCCACTGCCG15660.1399056217759616No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15530.1387442085683706No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15440.1379401532708076No Hit
AGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACC15400.1375827953607796No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA15130.13517062946809064No Hit
GGACTGGAGTGGGTGGCAGTTATTTCATACGATGGAAGCGATAAATATTA15110.1349919505130766No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14990.1339198767829926No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA14720.13150771089030364No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14430.12891686604260064No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14120.12614734223988366No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACTCTCC13650.12194838679705465No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGT13620.12168036836453365No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13620.12168036836453365No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13530.12087631306697066No Hit
GTCTCCAAGGCGCCTACTTTATAAGGTTTCTAACCGGGACTCTGGGGTCC13500.12060829463444965No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13330.11908952351683066No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACT13190.11783877083173266No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGACCATCACTGAACACAGA13090.11694537605666268No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13080.11685603657915566No Hit
GCAGTGGGTCAGGCACTGATTTCACACTCAAAATCAGCAGGGTGGAGGCT12800.11435453120895968No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12740.11381849434391768No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12470.11140632845122868No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12460.11131698897372169No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12210.10908350203604669No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12150.10854746517100469No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC11940.1066713361433577No Hit
GGCCTGGCCTCTGGTGAAACCAATTCAAGTGGATGTTTCCGTCGCTGTAT11940.1066713361433577No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11910.1064033177108367No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11790.1053312439807527No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11610.1037231333856267No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11580.1034551149531057No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11580.1034551149531057No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11290.10086427010540272No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11250.10050691219537472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2600.046.4218181
GTATCAA15100.043.619821
TGGTATC2850.042.3194582
TCTGGAG14850.041.58691470
CTCGTAT602.8456088E-740.36577639
TATCAAC17600.037.592872
GGTATCA6350.037.471651
CGTATGC753.8846338E-836.94396641
AATAGAC11200.034.7678073
ATCAACG19400.034.104873
TAGACAG11750.032.8471035
ATAGACA11750.032.8471034
TCAACGC20150.032.8354574
CAGCCGT11800.032.706469
CAACGCA20450.032.3537645
GTAATAG12200.032.2234841
AGCCGTG12000.032.1613510
TAATAGA12150.032.049342
AACGCAG20800.031.809356
AATTGTC12850.031.16329663
Produced by FastQC (version 0.11.5)