FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119326
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAGGTCTCAGACTCTTCATTTGCAGATACA40840.36486242613858694No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC39350.351550843990044No Hit
GTCTATTACTGTGCGACTCTTTCCGGGGTGGCAGTTATTTTCCCCTACCAGTACGACGGTTTGGACGTCTGGGGC38370.342795575194358No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38070.340115390869148No Hit
GTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT30400.2715920116212792No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGAA29660.2649808902857613No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC27250.2434500762065743No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26940.2406805524038573No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25670.22933443876046838No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23970.2141467275842784No Hit
GGTCTAGTCAGAGCCTCGAATACAGCGACGGAAACATCCACTTGAATTGGTTTCACCAGAGGCCAGGCCAGTCTC23410.2091437168438864No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22690.20271127446338244No Hit
GGTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT22640.20226457707584744No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22520.2011925033457634No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22340.19958439275063744No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22210.1984229795430464No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21660.19350930828016144No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21370.19091846343245847No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG21360.19082912395495147No Hit
AGGTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT19930.1780535786714505No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18820.16813689666817352No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAAGTAGGCGCCTTGGAGACTGGCCTGGCCTCTGGTGAAA18700.16706482293808952No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTCAA17920.16009634369254355No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17860.15956030682750155No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17110.15285984601447658No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17010.15196645123940658No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTGAGTGTGAAATCAGTGCCTGACCCACTGCCG16730.14946494586921058No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16700.14919692743668958No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16690.14910758795918258No Hit
ACTCTACACTGGGTCCGCCAGGCCCCAGGCAAGGGACTGGAGTGGGTGGC16000.1429431640111996No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15900.1420497692361296No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15890.14196042975862258No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15840.14151373237108758No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15680.1400843007309756No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15650.1398162822984546No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15470.1382081717033286No Hit
AGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACC15460.1381188322258216No Hit
GTGTAGAGTATAGCCACTAAAGGGGAATCCAGAACCTGCACAGGAGAGTC15380.1374041164057656No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGAATA15340.1370467584957376No Hit
CTCCAGTCCCTTGCCTGGGGCCTGGCGGACCCAGTGTAGAGTATAGCCAC15080.13472393208055564No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14720.13150771089030364No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTACTTTATAAGGTTTCTAACCGGGAC14710.1314183714127966No Hit
GGACTGGAGTGGGTGGCAGTTATTTCATACGATGGAAGCGATAAATATTA14640.13079299507024764No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14510.12963158186265664No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA14160.12650470014991164No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13970.12480725007727865No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGACCATCACTGAACACAGA13620.12168036836453365No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13560.12114433149949166No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13470.12034027620192865No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13200.11792811030923968No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13000.11614132075909968No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12910.11533726546153669No Hit
GTCTCCAAGGCGCCTACTTTATAAGGTTTCTAACCGGGACTCTGGGGTCC12850.11480122859649468No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12720.11363981538890368No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12660.11310377852386169No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT12590.11247840218131268No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12490.11158500740624268No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12470.11140632845122868No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12460.11131698897372169No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT12450.11122764949621469No Hit
GCAGTGGGTCAGGCACTGATTTCACACTCAAAATCAGCAGGGTGGAGGCT12320.11006623628862369No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12100.10810076778346969No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12010.1072967124859067No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACT12000.10720737300839969No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGT11980.10702869405338569No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11870.10604595980080869No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACACAC11840.1057779413682877No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11540.1030977570430777No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11460.1023830412230217No Hit
GCCTCCACCCTGCTGATTTTGAGTGTGAAATCAGTGCCTGACCCACTGCC11230.1003282332403607No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCACTCTCC11200.10006021480783972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1650.050.0150381
GTATCAA15550.044.88891
TATCAAC16900.041.088622
TCTGGAG12750.039.29210770
GGTATCA6100.038.8948941
ATCAACG18750.037.032893
TCAACGC19250.036.0709954
AACGCAG19350.036.0638436
CAACGCA19500.035.6085435
CGGACTA400.002616559234.4453724
AGAGTAC20300.034.238911
CAGAGTA21050.033.0189910
TAGACAG10350.032.8800435
GCAGAGT21250.032.708229
CGCAGAG21300.032.6183178
AATAGAC10450.032.56543
TGGTATC2550.032.3539962
CTCTGGA12200.031.63080869
ATAGACA10900.031.2209534
CAGCCGT11050.031.1388269
Produced by FastQC (version 0.11.5)