FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807879
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA31160.38570132408442354No Hit
GGTCTATACTTTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAAT23000.284696099292097No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19160.23716422880159035No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12500.15472614091961792No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12270.15187917992669694No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12270.15187917992669694No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11800.1460614770281193No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11690.14469988698802666No Hit
GCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACATCGGTT11200.13863462226397766No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11070.13702547039841362No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA10450.12935105380880058No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG9990.12365713182295865No Hit
GTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCATCACGATTCAGGAG9970.12340956999748726No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA9360.1158589343206099No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9070.11226928785127475No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8810.10905098412014672No Hit
CCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTC8610.10657536586543281No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA8500.10521377582534018No Hit
CCACTATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAA8210.10162412935600504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8160.10100522479232657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC451.8887404E-645.9002273
GTATCAA14500.040.12811
GGTATCA5900.039.0978281
TATCAAC16400.035.264812
CACGGAT3550.035.0628170
GTGGTAT2550.033.7543451
ATCAACG17300.033.2312363
CAACGCA17400.033.0402535
TCAACGC17300.033.0322464
AACGCAG18200.031.398786
CGAACTA553.258592E-431.35966329
TGTAACG553.2978988E-431.2956122
CTAGTAC450.004671236530.6001513
CAGAGTA19150.030.22873910
AGAGTAC19200.030.15001511
CGCAGAG19500.030.0339958
GTATAAG1950.030.0154041
ACGCAGA19250.029.8723497
GCAGAGT20600.028.0992479
TGGTATC3300.027.1228622
Produced by FastQC (version 0.11.5)