FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931314
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11890.12766907831300722No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTTAGGACCA11740.1260584507480828No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11140.11961594048838524No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10100.10844892270490941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15300.043.0330851
TCGTATG702.1122105E-839.5874440
GTACTAG350.00135316739.396031
ACGTAAT350.00135316739.396031
TATCAAC17550.037.4958762
ATCAACG18450.035.2933353
CAACGCA19000.034.453025
TCAACGC19200.034.094134
AACGCAG19250.033.82666
CTCGTAT652.2631113E-531.96411539
GTAACAC652.3427736E-531.8027863
CAGAGTA20550.031.34972610
AGAGTAC20850.030.73341411
TAACGGT450.004654382430.62326220
CGCAGAG21400.030.4248668
ACGCAGA22250.029.2625687
GTATTAG1551.8189894E-1228.9116021
GCAGAGT22650.028.8978829
CGTATGC1103.493733E-828.36397641
GTGGTAT2700.028.087911
Produced by FastQC (version 0.11.5)