FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931314
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11260.12090444254032474No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10760.11553568399057675No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10450.112207053689733No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10400.1116701778347582No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT10330.11091855163779349No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTTAGGACCA10280.11038167578281868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17650.044.656541
TATCAAC19950.039.312412
TCAACGC21450.036.400974
CAACGCA22000.035.9599885
ATCAACG22050.035.5683673
AACGCAG23050.034.3200576
GTGGTAT2450.032.3114171
AGAGTAC24300.032.00907511
CAGAGTA24550.031.68311710
ACGCAGA25500.030.4863877
GCAGAGT25900.030.2974479
CGCAGAG25700.030.2588868
CGAAATA953.2541902E-728.99973724
CACACGT1406.4369786E-527.4201470
GGTATCA11150.026.5471481
CTAGACA1057.884955E-726.2068654
AGTACGG24550.025.65491313
GAGTACG25450.024.882912
GCTAGAC700.001356781924.5702573
TACGGGT1701.72804E-1024.29423715
Produced by FastQC (version 0.11.5)