FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247325
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17500.042.2508131
GTATCAA41650.038.97281
TATCAAC49350.032.947092
GTGGTAT5950.032.945231
ATCAACG51300.031.9627463
CAACGCA52350.031.4529885
TCAACGC52350.031.4529884
AACGCAG53100.031.138216
TGGTATC6200.031.0484142
CTTATCG850.005810966229.26313270
ACGCAGA56950.028.9728077
CGCAGAG58000.028.44838
AGAGTAC56000.028.29801811
CAGAGTA56450.028.13332710
GCAGAGT60150.027.4885949
TCGTATG2700.026.976240
CGTATGC2800.026.02539841
CTCGTAT2450.025.46335439
TATACAC3000.025.2083553
TTAACGG3850.023.33779735
Produced by FastQC (version 0.11.5)