FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247325
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27250.21846752049385684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8350.042.728421
TGGTATC9150.039.353332
GTATCAA44100.035.7062341
GGTATCA20950.035.534131
CGCCTTA653.1545243E-431.53479269
TATCAAC52900.029.8853592
ATCAACG53250.029.495733
AACGCAG53550.029.4585696
TCAACGC53800.029.2579354
CAACGCA53950.029.1130225
ACGCAGA57100.027.5758637
CGCAGAG58450.026.7671788
AGAGTAC58200.026.474811
CAGAGTA58550.026.43376210
GCAGAGT60500.025.8643389
AGGTACG1103.4220342E-521.823212
AGTACGG61150.021.77431913
GAGTACG62500.021.35890212
GTGTTAG2051.8681021E-920.0815561
TACGCTG900.005936946719.0520065
Produced by FastQC (version 0.11.5)