FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055980
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACGATCACCAGTAGTATCCCACAC40020.37898445046307694No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACGATCACCA36910.3495331350972556No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32110.3040777287448626No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG27500.26042159889391847No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT24970.23646281179567794No Hit
CCCCAGGACAGACGGCCAGGTTTACCTGTGGGACAAACAACGTTGGAAGT23990.22718233299873106No Hit
GTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGAC23710.22453076762817478No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23210.2197958294664672No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22930.21714426409591092No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22560.21364040985624727No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21550.2040758347695979No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG21440.20303414837402226No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21400.2026553533210856No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19920.1886399363624311No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19830.18778764749332374No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19800.18750355120362128No Hit
CACCAGTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG19510.18475728706983086No Hit
GCACAGGGGCCTGGCCTGGCTTCTGTTGGTACCAGTGCACACTTTTACTT19180.18163222788310385No Hit
GGTTTACCTGTGGGACAAACAACGTTGGAAGTAAAAGTGTGCACTGGTACCAACAGAAGCCAGGCCAGGCCC19120.18106403530369894No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT18750.1775601810640353No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18450.17471921816701075No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17890.16941608742589823No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGA17750.1680903047406201No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17240.16326066781567833No Hit
GGGTGGCCGTGTTCCCAGACTTGGAGCCAGAGATTCGGTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA17110.16202958389363434No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC17030.16127199378776114No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16920.16023030739218547No Hit
GTCTCTGGTGCCTCCAGCAGGAGTTTCTACTGGAGCTGGATCCGGCAGCC16920.16023030739218547No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16850.15956741604954638No Hit
GCCCTCAGGGATCCCTGACCGAATCTCTGGCTCCAAGTCTGGGAACACGG16730.15843103089073657No Hit
CTACTGGTGATCGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA16630.15748404325839505No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG16380.15511657417754124No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16340.15473777912460462No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16170.15312790014962405No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA15980.15132862364817515No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA15700.1486770582776189No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGATTAT15340.14526790280118942No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACGATCACCAG15320.1450785052747211No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15190.14384742135267714No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG15050.142521638667399No Hit
ATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGACCCTGC14950.14157465103505748No Hit
GGATACTACTGGTGATCGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14730.13949127824390614No Hit
GTATATTACTGTGCGAGAGGGATTTTATCTAGTGACCTCCCGGGGAACTGGTTCGACCCCTGGGGCCAGGGAACC14610.1383548930850963No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14570.13797609803215968No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC14400.1363662190571791No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14380.1361768215307108No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGACCG14160.13409344873955947No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13910.13172597965870567No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13760.13030549821019338No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13680.12954790810432015No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA13580.12860092047197863No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13560.12841152294551034No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGTTTAC13510.12793802912933958No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13450.12736983654993464No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC13240.12538116252201745No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC13210.125097066232315No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG13130.12433947612644179No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT12780.12102501941324646No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12740.12064622436030986No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12740.12064622436030986No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC12690.1201727305441391No Hit
ATCACCAGTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGG12640.11969923672796833No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12610.11941514043826587No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12530.11865755033239267No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12350.11695297259417792No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12230.11581658743536809No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11780.11155514308983125No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11770.1114604443265971No Hit
GCCGAATACCACACGATCACCAGTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGACC11750.1112710468001288No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11700.11079755298395803No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11690.11070285422072389No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11650.11032405916778726No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11550.10937707153544575No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11520.1090929752457433No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG11470.10861948142957252No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11420.10814598761340177No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11370.10767249379723101No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11330.10729369874429441No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11060.10473683213697228No Hit
GTAATAATCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG11030.10445273584726983No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11020.10435803708403568No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10900.10322165192522585No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC10870.1029375556355234No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10790.10217996552965018No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10750.10180117047671358No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10720.10151707418701113No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC10710.10142237542377698No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAGGTTTACCTGTGGGACAAACAA10710.10142237542377698No Hit
CCTGAGGGCCGGTCGCTATCATCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGTTGGTACCAGTGCACA10680.1011382791340745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG150.002298135569.4302646
CTAAATT200.00726176651.92413740
ATCATAC200.00736194551.7445261
TATAACA200.007381274451.7102242
AATCGTT459.840921E-538.44769739
TTATACA655.483598E-737.125292
GGTATCA7350.035.2003561
AATAGCG501.7005752E-435.03421862
CGTATGC709.458108E-734.62761741
CTCTTAT501.8624726E-434.4963531
TACGGAG601.34953225E-534.4734842
TATACAC807.141534E-834.473483
TATCAAC15500.032.6942062
GTCAGAT958.860297E-932.659096
CATTGAT6100.032.65138270
TGGATTG7900.032.53138770
TCGTATG751.6258473E-632.30835340
CTTATAC751.6704416E-632.1965941
AGGGGGG1401.697992E-632.1247570
ACCTTTA553.0440002E-431.72714256
Produced by FastQC (version 0.11.5)