FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055980
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACGATCACCAGTAGTATCCCACAC38200.36174927555446124No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACGATCACCA33600.3181878444667513No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30950.2930926722097009No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGA26100.24716377204113713No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG25890.24517509801321993No Hit
CCCCAGGACAGACGGCCAGGTTTACCTGTGGGACAAACAACGTTGGAAGT23660.224057273812004No Hit
GTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGAC23170.21941703441353053No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT22710.21506089130475955No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21890.20729559271955908No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21780.20625390632398338No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20910.19801511392261217No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20610.1951741510255876No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19990.18930282770507018No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19850.18797704501979204No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19730.1868406598609822No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG19530.18494668459629915No Hit
GGGTGGCCGTGTTCCCAGACTTGGAGCCAGAGATTCGGTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA19410.18381029943748936No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19000.17992765014488912No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18960.1795488550919525No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT18430.17452982064054243No Hit
GGTTTACCTGTGGGACAAACAACGTTGGAAGTAAAAGTGTGCACTGGTACCAACAGAAGCCAGGCCAGGCCC18390.17415102558760584No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18280.17310933919203014No Hit
GCACAGGGGCCTGGCCTGGCTTCTGTTGGTACCAGTGCACACTTTTACTT17740.16799560597738594No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC17560.1662910282391712No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17390.1646811492641906No Hit
CACCAGTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG17220.16307127028921004No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16850.15956741604954638No Hit
GTCTCTGGTGCCTCCAGCAGGAGTTTCTACTGGAGCTGGATCCGGCAGCC16680.1579575370745658No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16440.15568476675694615No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG16030.15180211746434594No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16010.15161271993787762No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG15880.15038163601583363No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA15760.1492452508570238No Hit
GCCCTCAGGGATCCCTGACCGAATCTCTGGCTCCAAGTCTGGGAACACGG15550.14725657682910662No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC15490.1466883842497017No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA15330.14517320403795525No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14940.14147995227182333No Hit
CTACTGGTGATCGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA14860.14072236216595013No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACGATCACCAG14850.14062766340271596No Hit
GGATACTACTGGTGATCGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14840.1405329646394818No Hit
GTATATTACTGTGCGAGAGGGATTTTATCTAGTGACCTCCCGGGGAACTGGTTCGACCCCTGGGGCCAGGGAACC14620.13844959184833044No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGATTAT14500.13731320668952063No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG13890.13153658213223735No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13880.1314418833690032No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGTTTAC13860.1312524858425349No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13220.12519176499554915No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13160.12462357241614425No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGACCG13150.1245288736529101No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13130.12433947612644179No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13100.12405537983673934No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13030.12339248849410026No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT12990.12301369344116365No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC12700.12026742930737325No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12540.11875224909562682No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA12340.11685827383094377No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC12200.11553249114566563No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12180.11534309361919734No Hit
ATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGACCCTGC12110.11468020227655827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12060.1142067084603875No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11980.1134491183545143No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11960.11325972082804599No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11850.1122180344324703No Hit
GCCGAATACCACACGATCACCAGTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGACC11790.1116498418530654No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11790.1116498418530654No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11570.10956646906191406No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11550.10937707153544575No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11490.10880887895604084No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11450.10843008390310421No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11420.10814598761340177No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11420.10814598761340177No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG11160.10568381976931382No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11060.10473683213697228No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11040.10454743461050398No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10950.10369514574139663No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10940.10360044697816247No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC10890.10312695316199169No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10870.1029375556355234No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG10750.10180117047671358No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10740.10170647171347943No Hit
ATCACCAGTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGG10730.10161177295024527No Hit
GGTACCAACAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGAT10650.10085418284437205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)