FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064272
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.040.8913651
GTATCAA21700.038.3382571
GTGGTAT3050.034.9412651
TATCAAC25800.032.2366562
ATCAACG27500.030.368823
CAACGCA27450.030.173735
TCAACGC28100.029.842684
AACGCAG28800.028.6400036
TGGTATC3250.028.551882
AGAGTAC30250.026.69911211
TCGTATG658.489752E-426.62707540
ACGCAGA31100.026.5219337
CAGAGTA31300.025.91325810
CGCAGAG32850.025.1090458
TATAAGA2950.024.465362
GCAGAGT34100.024.2894129
CTCGTAT1052.2282304E-523.0691639
GTATACC1103.3573815E-521.8767171
GTATAGG953.139892E-421.7122291
GTATAAG3800.021.7122291
Produced by FastQC (version 0.11.5)