FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100348
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36940.33571197475707687No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTACACGATCACCAGTAGTATCCCACAC36760.33407612864293845No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTACACGATCACCA34400.31262836847979003No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29350.26673379694423943No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG29070.2641891474333575No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28710.2609174552050806No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25020.2273826098652426No Hit
GGATTACCTGTGGGACAAACAACGTTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC24820.22556500307175548No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24030.21838545623748123No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGACCCCT23970.21784017419943508No Hit
GTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGCCCTCGAC23560.21411408027278644No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23550.21402319993311208No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGACAAACAACGTTGGAAGT23220.21102414872385825No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21830.1983917815091226No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21810.19821002082977385No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21270.19330248248735854No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG21000.1908487133161509No Hit
ACCCTACTGATGGTCAGGGTGGCCGTGTTCCCAGACTTGGAGCCAGAGAT20530.1865773373514561No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT20400.18539589293568942No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20340.1848506108976433No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19420.1764896196476024No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18740.17030975654974606No Hit
CTACTGGTGATCGTGTAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA18190.16531133786765642No Hit
GGGTGGCCGTGTTCCCAGACTTGGAGCCAGAGATTCGGTCAGGGGTCCCTGAGGGCCGGTCGCTATCATCATAGA17930.16294844903612313No Hit
CACCAGTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGCCCTCGACCCTACTGATGGTCAGGGTGG17820.16194876529970517No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17720.16103996190296163No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC16770.15240632963389764No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16750.15222456895454892No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16540.15031608182138742No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16510.15004344080236434No Hit
GGATACTACTGGTGATCGTGTAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC16250.14768055197083105No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT16190.14713526993278492No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTACACGATCACCAG16070.14604470585669263No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15750.14313653498711315No Hit
CAGTAGGGTCGAGGGCGGGGATGAGGCCGATTATTACTGTCAGGTGTGGGATACTACTGGTGATCGTGTAGTATT15670.1424094922697183No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG15550.14131892819362601No Hit
CGGCCACCCTGACCATCAGTAGGGTCGAGGGCGGGGATGAGGCCGATTAT15470.14059188547623117No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15370.13968308207948757No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA15210.13822899664469784No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG15210.13822899664469784No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC14990.136229629171862No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14910.1355025864544671No Hit
GCCCTCAGGGACCCCTGACCGAATCTCTGGCTCCAAGTCTGGGAACACGG14730.1338667403403287No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGACCCCTGACCG14690.13350321898163128No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14650.13313969762293384No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14640.13304881728325946No Hit
ATACTACACGATCACCAGTAGTATCCCACACCTGACAGTAATAATCGGCC14480.13159473184846976No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14360.13050416777237747No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14240.12941360369628518No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14170.12877744131856467No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA14160.1286865609788903No Hit
ATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGCCCTCGACCCTAC13740.12486958671256729No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13290.1207799714272212No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13120.11923500565275713No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13100.11905324497340841No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12920.11741739885926998No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG12500.11360042459294696No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC12280.11160105712011109No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA12260.11141929644076239No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC12060.10960168964727522No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12000.10905640760922906No Hit
GTAATAATCGGCCTCATCCCCGCCCTCGACCCTACTGATGGTCAGGGTGG11800.10723880081574193No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11690.106239117079324No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11540.10487591198420863No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11490.10442151028583684No Hit
GTGTAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC11270.10242214281300098No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11090.10078629669886255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20300.041.980211
GGTATCA8250.041.2353941
CGTATGC1550.038.0080141
TATCAAC22700.037.213632
TCGTATG1400.037.08885240
ATCAACG24250.034.835033
TCTCGTA1500.034.5592738
GTTAGCC701.0097501E-634.3394053
TCAACGC24800.034.0624774
CAACGCA24900.033.925685
AACGCAG25500.033.127436
CGCAGAG26500.032.006928
AGAGTAC27350.030.6355211
ACGCAGA27900.030.4008397
CAGAGTA27700.030.372410
GCAGAGT28000.030.1696229
TATACGG3150.028.343645
TTATACA2250.027.4715272
GTGGTAT3150.027.2720891
ACGAGAC2050.026.81123522
Produced by FastQC (version 0.11.5)