FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100348
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35780.3251698553548514No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTACACGATCACCAGTAGTATCCCACAC32310.2936343774878493No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTACACGATCACCA30220.2746403864959086No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG27880.2533743870121089No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27470.24964829308546024No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27010.24546779746043978No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25630.23292631058537844No Hit
GGATTACCTGTGGGACAAACAACGTTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC24980.2270190885065452No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGACCCCT24040.2184763365771556No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23260.2113876700825557No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22000.19993674728358665No Hit
GTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGCCCTCGAC21820.1983009011694482No Hit
GGGTGGCCGTGTTCCCAGACTTGGAGCCAGAGATTCGGTCAGGGGTCCCTGAGGGCCGGTCGCTATCATCATAGA21450.19493832860149699No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGACAAACAACGTTGGAAGT21110.19184839705256881No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20470.18603205531340994No Hit
ACCCTACTGATGGTCAGGGTGGCCGTGTTCCCAGACTTGGAGCCAGAGAT20300.18448708953894585No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT20140.18303300410415616No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19940.181215397310669No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19830.18021571357425106No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18610.16912831213397944No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG18440.16758334635951536No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18280.16612926092472563No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17990.16349373107416929No Hit
CTACTGGTGATCGTGTAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA17470.15876795341110267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17430.15840443205240523No Hit
CACCAGTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGCCCTCGACCCTACTGATGGTCAGGGTGG17090.1553145005034771No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC16990.15440569710673352No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16680.15158840657682843No Hit
GGATACTACTGGTGATCGTGTAGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC16380.1488619963865977No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16370.14877111604692334No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT16200.14722615027245925No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16120.1464991075550644No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16020.1455903041583208No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15780.14340917600613623No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG15750.14313653498711315No Hit
CAGTAGGGTCGAGGGCGGGGATGAGGCCGATTATTACTGTCAGGTGTGGGATACTACTGGTGATCGTGTAGTATT15670.1424094922697183No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15620.14195509057134653No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC15050.13677491120990815No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC14900.13541170611479278No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG14830.13477554373707226No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14790.13441202237837482No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14760.13413938135935177No Hit
GCCCTCAGGGACCCCTGACCGAATCTCTGGCTCCAAGTCTGGGAACACGG14560.1323217745658646No Hit
CGGCCACCCTGACCATCAGTAGGGTCGAGGGCGGGGATGAGGCCGATTAT14560.1323217745658646No Hit
ATACTACACGATCACCAGTAGTATCCCACACCTGACAGTAATAATCGGCC14360.13050416777237747No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTACACGATCACCAG14350.1304132874327031No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA14250.12950448403595952No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14240.12941360369628518No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14160.1286865609788903No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGACCCCTGACCG13930.1265963131663801No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA13740.12486958671256729No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13720.12468782603321858No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12600.11450922798969053No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA12510.1136913049326213No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12500.11360042459294696No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12220.11105577508206495No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG12020.1092381682885778No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12010.10914728794890344No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11870.10787496319346243No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC11780.10705704013639322No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11740.10669351877769578No Hit
GTAATAATCGGCCTCATCCCCGCCCTCGACCCTACTGATGGTCAGGGTGG11140.10124069839723432No Hit
ATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGCCCTCGACCCTAC11110.10096805737821125No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11060.10051365567983947No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC11050.10042277534016511No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT11010.10005925398146769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)