FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017746
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC45740.44942451259941085No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC35480.3486135047447988No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT33900.3330890025605603No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29930.2940812344140876No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28360.2786549885727873No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC26180.2572351058122557No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGACTGTCATCCCATG25340.24898157300544538No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC24680.24249665437152296No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGA24430.2400402457980675No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23630.23217973836301004No Hit
ATAATAATCAGCCTCATCCCCAGACTGGAGTCCACTGATGGCCAGGGAGG23190.2278564592737284No Hit
GCCCTGAGGGCCGCTGATTATTCCTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGGTCA23180.2277582029307902No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23070.2266773831584698No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22760.22363143652738504No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22560.22166630966862066No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22530.221371540639806No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22240.21852210669459768No Hit
GTAATAGAGGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA21300.20928601045840511No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20490.20132724668040944No Hit
CCTCAGGGCTCCCTGACCGGTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGT20400.2004429395939655No Hit
GATTATTATTGTGCAGCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTA20380.20024642690808905No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19230.18894694747019394No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG18340.1802021329486925No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17810.17499454677296694No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17690.1738154706577083No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17610.17302941991420256No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17550.17243988185657325No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17420.1711625493983764No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17340.17037649865487067No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17150.16850962813904452No Hit
GCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGG17020.1672322956808477No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16950.16654450128028014No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16940.16644624493734195No Hit
GTCATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTC16920.1662497322514655No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16650.1635968109921336No Hit
GGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCC16270.1598630699604813No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16260.15976481361754308No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGGTCACAGAATTACCG15770.15495025281357036No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15750.15475374012769394No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA15740.15465548378475574No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15600.15327989498362066No Hit
TCCCCAGACTGGAGTCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGAGC15240.1497426666378448No Hit
ATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTCCAC15130.14866184686552442No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG14900.1464019509779454No Hit
CTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCTGTGACCTGGTACCAGCAACTCCCAGGAACGGCCCC14880.14620543829206895No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG14810.14551764389150143No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT14600.14345426068979883No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14480.14227518457454022No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14460.14207867188866377No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA14450.14198041554572555No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC14280.14031005771577584No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14230.13981877600108475No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14070.13824667451407324No Hit
CTGTCATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAG14050.13805016182819682No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGTCTGGGGATG13970.13726411108469108No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13830.13588852228355602No Hit
GTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGAGCCCTGAGGGCCGCTG13830.13588852228355602No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13820.1357902659406178No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGAC13660.1342181644536063No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13530.13294083199540946No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13520.13284257565247126No Hit
ACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGAC13430.13195826856602727No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG13360.13127047416545976No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT13220.1298948853643247No Hit
GTCTGGGGATGAGGCTGATTATTATTGTGCAGCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGAC13180.12950185999257183No Hit
CTCTATTACTGTGCGAGAAGCATAACTGGGGATCCAGGCTACTGGGGCCC13130.12901057827788073No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC13090.12861755290612786No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12700.12478555553153733No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT12290.12075704547107038No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCTGTGAC12150.11938145666993533No Hit
TGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCC12040.11830063689761493No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCT11960.11751458615410917No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11890.11682679175354166No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTC11870.11663027906766521No Hit
GTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC11870.11663027906766521No Hit
ACACCGGACCATTCAGACTGTCATCCCATGCTGCACAATAATAATCAGCC11790.11584422832415947No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG11770.11564771563828302No Hit
GTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGAC11700.11495992123771549No Hit
GCCCCCAAACTCCTCATTTATAGGAATAATCAGCGGCCCTCAGGGCTCCCTGACCGGTTCTCTGGCTCCAAGT11610.11407561415127153No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11580.11378084512245687No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC11570.11368258877951867No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTC11500.11299479437895113No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11500.11299479437895113No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11250.11053838580549566No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC11210.1101453604337428No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC11190.10994884774786637No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC11160.1096540787190517No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11140.10945756603317527No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC11140.10945756603317527No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11140.10945756603317527No Hit
GATTATTCCTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGG10830.1064116194020905No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10830.1064116194020905No Hit
GGCCATCAGTGGACTCCAGTCTGGGGATGAGGCTGATTATTATTGTGCAG10740.10552731231564653No Hit
CAGTAATAGAGGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGATTCTTCGACATGTCTA10650.10464300522920256No Hit
GTGGACTCCAGTCTGGGGATGAGGCTGATTATTATTGTGCAGCATGGGAT10580.10395521082863504No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10510.10326741642806751No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10510.10326741642806751No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA10460.10277613471337643No Hit
ATTCAGACTGTCATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAG10430.10248136568456177No Hit
CTATTACTGTGCGAGAAGCATAACTGGGGATCCAGGCTACTGGGGCCCGGGAGCCCTGGTCAGCGTCTCCTCAGC10260.10081100785461206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTAT350.005106268556.75449470
TAACCGA200.007278626351.89353639
TGGTATC2650.042.73642
GTATCAA16100.042.008871
GGTATCA6550.041.4082071
GTGGTAT2850.039.7529831
CTGTTTA859.111633E-538.94915870
TATAAGA1700.038.356112
ATAAGAG1700.038.354233
GTATAAG1800.038.1468051
TATCAAC17950.037.4732632
ATAGTTC400.002664814234.316943
TAAGAGA1900.034.316944
ATCAACG20300.033.4716953
TCAACGC20650.032.7381944
AACGCAG20800.032.6670886
CAACGCA20850.032.424165
AAGAGAC2200.031.1972185
CAGAGTA22650.030.16376310
AGAGTAC22600.030.07858511
Produced by FastQC (version 0.11.5)