FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545518
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT8540.15654845486308425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2400.043.9829770
TGGTATC3050.043.9314882
GTGGTAT3300.040.625691
GGTATCA7550.037.335071
GTATCAA18000.037.0492441
CTTATAC1750.035.3577421
TCGTATG1600.034.6809540
TTATACA1700.034.3566782
CGTATGC1650.033.6486941
CTCGTAT1550.033.5404839
TATCTCG1600.032.3934936
TATCAAC21250.031.3656252
ATCAACG21500.030.841113
ACGGACT1800.030.5672927
TCAACGC21850.030.5043264
TATACAC1950.029.9519753
CAACGCA22250.029.8015235
AACGCAG22900.028.9556276
AGTCTAG500.007502502327.77045642
ACGCAGA24050.027.711377
Produced by FastQC (version 0.11.5)