FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626030
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18650.2979090458923694No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15760.25174512403558935No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14080.22490934939220164No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13550.21644330143922816No Hit
GTTACAATACCCCTCGCGAGCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT13240.21149146207050779No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13230.21133172531667813No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12570.20078909956391866No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11970.19120489433413734No Hit
GAGTTACAATACCCCTCGCGAGCTCACCTTCGGCGGAGGGACCAAGGTGG10800.17251569413606377No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10550.16852227529032154No Hit
ACAATACCCCTCGCGAGCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT10440.166765170998195No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT10280.16420938293691995No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10270.16404964618309026No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10170.1624522786447934No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9340.14919412807692922No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTGAGCTCGCGAGGGGTATTGTAACT9170.14647860326182452No Hit
GGGTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT8970.14328386818523073No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAA8820.1408878168777854No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8710.13913071258565884No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8690.13881123907799947No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8570.1368943980320432No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAACTATTTAAATTGGTATCAGCAGAAA8380.1338593997092791No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8340.13322045269396035No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8170.13050492787885565No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8050.12858808683289938No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7900.12619203552545405No Hit
ACTTACTACTGTCAACAGAGTTACAATACCCCTCGCGAGCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAA7780.12427519447949778No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7720.12331677395651965No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7640.12203887992588214No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7460.11916361835694776No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7420.118524671341629No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA7350.11740651406482118No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACC7250.1158091465265243No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7190.11485072600354615No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7070.1129338849575899No Hit
GTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGATTTCACT7020.11213520118844145No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT7000.11181572768078206No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6830.10910020286567736No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6760.10798204558886955No Hit
GGGTACGACAGGCCCCTGGACAAGGGCTCGAGTGGATGGGAGTAATCAACCCTAGTGGGGGTACCACAAGATACG6740.10766257208121018No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACCCCTCGCGAGCT6650.10622494129674297No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6640.10606520454291327No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6570.10494704726610546No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6550.10462757375844609No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6310.10079389166653355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAACG100.009998005129.2422270
GATCTAA200.00743288251.6166925
GTGGTAT1450.042.618311
GTATCAA9300.038.392121
TATCAAC11150.032.327452
ATCTAAA450.004703640530.55586616
CAACGCA12350.029.1863235
AACGCAG12400.028.791796
ATCAACG12550.028.72123
TCAACGC12550.028.72124
GGGAACG2104.3874024E-827.69476170
GTATATA500.007919311527.4651341
GTATAGT658.907873E-426.4066736
AGAGTAC13300.026.09624111
ACGCAGA13700.026.0701357
CGCAGAG13600.026.0217868
TATAAGA951.09109515E-525.2948112
TGCTTTA1551.5916157E-924.38760814
CAGAGTA14800.023.91573510
TGTTAGA3603.092282E-1123.335470
Produced by FastQC (version 0.11.5)