FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153060
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25640.22236483790956238No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23020.19964268988604236No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21610.1874143583161327No Hit
GTAATAAAGAGCCGTGTCTGCGGCGGTCATAGAGTTCAGGCTCAGGGAGA20440.17726744488578217No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19560.16963557837406554No Hit
CTTTATTACTGTGCGACACATAGCCAACTGGGAATTAACTACTGGGGCCA17850.15480547412970705No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT17510.15185679843199834No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16210.14058245017605328No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15960.13841430628067924No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15650.1357258078504154No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15450.13399129273411617No Hit
GTCTCTGGTGTCTCCATCAGTAGGAGTAGTTACTACTGGGGCTGGATCCG15360.13321076093178152No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15180.1316496973271122No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15070.1306957140131476No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14940.12956827918755312No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT14600.1266196034898444No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14470.12549216866424992No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14330.12427800808284045No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14000.12141605814094669No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13700.11881428546649785No Hit
GAGTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG13680.11864083395486792No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCAGTAGGAGTAGTTACTACTGGG13470.11681959308275372No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13420.11638596430367891No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13250.11491162645482454No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12880.11170277348967098No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12830.11126914471059615No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12670.10988153261755676No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12630.10953462959429693No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12440.10788684023381263No Hit
TCTATGATGCGTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT12250.10623905087332837No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12100.10493816453610394No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11990.10398418122213936No Hit
GTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG11930.10346382668724957No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCTCACTTTCGGCGGAGGGA11780.10216294035002514No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCTACGTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCACGCT11700.10146913430350546No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11620.10077532825698576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17750.039.1472361
TCGTATG801.5788828E-938.96446240
TATCAAC20300.034.04552
GGTATCA7800.033.0761341
ATCAACG21800.031.3874683
TCAACGC22700.030.143034
CAACGCA22800.029.709215
AACGCAG23250.029.4299746
TACGGAT9150.028.6297870
CGTATGC1004.812282E-727.7322941
AGAGTAC24900.027.20597311
AGTTAGT1407.6581484E-526.73086470
CGCAGAG26150.025.9043738
GCAGAGT26600.025.5965219
CAGAGTA26900.025.31105810
AGTACGG21300.024.8621413
ACGCAGA27500.024.756677
TATATAC700.00136022224.5601864
TATACAC1551.5861588E-924.4017353
GAGTACG22100.023.80655312
Produced by FastQC (version 0.11.5)