FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224400
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT4660.20766488413547238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCTA200.00669560152.9867655
TATCCCG200.007631344351.2630355
GTGGTAT1650.043.5251
TGGTATC1600.042.7191932
AGGGGGG1052.1271444E-741.83824570
GTATCAA9150.041.112611
TTATACA759.495125E-1041.010432
TATACAC759.495125E-1041.010433
ATACACA857.2759576E-1140.2063034
TACACAT955.456968E-1239.571475
GTGTAAG350.00140488139.0836751
CTTATAC801.7753337E-938.4729921
CTCGTAT852.8703653E-936.62636639
TATCAAC10300.036.4979552
CAACGCA10800.034.808235
TCGTATG904.98585E-934.61498640
AACGCAG10900.034.488896
CTCTTAT400.002705998534.1982151
ATCAACG11100.033.867473
GGTATCA3650.033.7297481
Produced by FastQC (version 0.11.5)