FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693745
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42440.6117521567723011No Hit
GCCTTGGGCTGACCCAGGACGATCAGTTTGGTCCCTCCGCCGAACACCCA40790.587968201572624No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23480.33845288975055676No Hit
GTCTGGGGGCCGTGCCTGGGAACTGCTGGTACCAGTATACATAATTAATG19870.2864164786773238No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19750.28468673648098364No Hit
GCCCCCAGACTCCTCATTTATGGGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT19140.27589388031625456No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18530.2671010241515254No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC18460.266092007870327No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18200.26234423311159No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAGTTTGGTCCCTC17960.2588847487189097No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16990.2449026659651601No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16420.2366863905325444No Hit
ATAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG15620.22515477589027671No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15380.22169529149759637No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT14720.21218170941772552No Hit
GATTATTATTGTGCAACATTGGATGACAGTCTGACTAGTTGGGTGTTCGG14580.2101636768553287No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14180.2043978695341948No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14060.2026681273378547No Hit
CCCCTGAGGGCCGCTGATTAGTCCCATAAATGAGGAGTCTGGGGGCCGTGCCTGGGAACTGCTGGTACCAGTATA13590.19589330373552244No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13510.19474014227129566No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13500.1945959970882673No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA13170.18983920604833188No Hit
ACATTGGATGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGACCAAACTGATCGTCCTGGGTCAGCCCAAGG13140.18940677049924684No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA12980.1871004475707933No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12650.18234365653085788No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT12560.18104634988360277No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12480.17989318841937602No Hit
GACCCAGGACGATCAGTTTGGTCCCTCCGCCGAACACCCAACTAGTCAGA12160.17528054256246892No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11750.16937059005830674No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11670.16821742859407995No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11450.16504623456745635No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG11390.16418136346928627No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11380.16403721828625792No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC11360.1637489279202012No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10740.15481192657244375No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10660.153658765108217No Hit
GTCATCCAATGTTGCACAATAATAATCAGCCTCATCCTCGGACCGGAGCC10630.15322632955913196No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC10400.14991099034948No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10240.14760466742102646No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10240.14760466742102646No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTATTGTGCAA10180.1467397963228564No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10060.1450100541265162No Hit
GTTTGGTCCCTCCGCCGAACACCCAACTAGTCAGACTGTCATCCAATGTTGCACAATAATAATCAGCCTCATCCT9770.14082984381869418No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9750.1405415534526375No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9720.14010911790355246No Hit
GGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG9560.13780279497509892No Hit
ATCCAATGTTGCACAATAATAATCAGCCTCATCCTCGGACCGGAGCCCAC9550.13765864979207057No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9540.13751450460904222No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG9440.13607305277875875No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9300.13405502021636193No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9280.13376672985030522No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9250.13333429430122018No Hit
TTGGATGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGACCAAACTGATCGTCCTGGGTCAGCCCAAGGCTGCC9210.13275771356910682No Hit
GATTAGTCCCATAAATGAGGAGTCTGGGGGCCGTGCCTGGGAACTGCTGG9130.13160455210488003No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9000.12973066472551154No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8730.12583874478374618No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT8710.1255504544176895No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC8690.12526216405163282No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAGTTTGGTCCCTCCGCCGAACACCCAA8590.12382071222134935No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8460.12194682484198084No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8430.1215143892928958No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8400.12108195374381076No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8250.11891977599838557No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC8200.11819905008324384No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8190.11805490490021549No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8110.11690174343598873No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAG7960.11473956569056354No Hit
ACCCAGGACGATCAGTTTGGTCCCTCCGCCGAACACCCAACTAGTCAGAC7950.11459542050753518No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC7810.11257738794513836No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7710.1111359361148549No Hit
GACTAGTTGGGTGTTCGGCGGAGGGACCAAACTGATCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT7680.11070350056576984No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG7610.1096944842845714No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG7480.10782059690520292No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG7480.10782059690520292No Hit
GTCCGAGGATGAGGCTGATTATTATTGTGCAACATTGGATGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGAC7440.10724401617308954No Hit
AATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGG7360.10609085470886276No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAGTTTGGTC7290.10508183842766435No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7160.10320795104829585No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7100.10234307995012576No Hit
ATGTATACTGGTACCAGCAGTTCCCAGGCACGGCCCCCAGACTCCTCATT6990.10075748293681396No Hit
GGACCAAACTGATCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG6940.10003675702167222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)