FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693745
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41260.5947430251749563No Hit
GCCTTGGGCTGACCCAGGACGATCAGTTTGGTCCCTCCGCCGAACACCCA30360.43762477567405894No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC24610.3547412954327599No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23040.33211050169730955No Hit
GTCTGGGGGCCGTGCCTGGGAACTGCTGGTACCAGTATACATAATTAATG21860.3151013700999647No Hit
GCCCCCAGACTCCTCATTTATGGGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT19920.2871372045924655No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT19180.2764704610483679No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18570.2676776048836388No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC17900.25801987762073963No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17580.25340723176383256No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17300.24937116663903883No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16440.23697468089860108No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15750.2270286632696452No Hit
GATTATTATTGTGCAACATTGGATGACAGTCTGACTAGTTGGGTGTTCGG14740.21246999978378223No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14700.2118934190516688No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAGTTTGGTCCCTC14310.20627175691356336No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC13830.1993527881282027No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT13590.19589330373552244No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13260.191136512695587No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13190.19012749641438856No Hit
CCCCTGAGGGCCGCTGATTAGTCCCATAAATGAGGAGTCTGGGGGCCGTGCCTGGGAACTGCTGGTACCAGTATA13060.1882536090350201No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12730.18349681799508466No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12690.18292023726297127No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12650.18234365653085788No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12500.18018147878543267No Hit
ACATTGGATGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGACCAAACTGATCGTCCTGGGTCAGCCCAAGG12260.17672199439275238No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12110.1745598166473272No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA11930.17196520335281695No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG11780.16980302560739177No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11390.16418136346928627No Hit
GGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG10880.15682995913484063No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10820.15596508803667053No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10620.15308218437610363No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10490.1512082969967351No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTATTGTGCAA10400.14991099034948No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10210.14717223187194142No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG9730.14025326308658081No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9560.13780279497509892No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9520.13722621424298553No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9420.13578476241270207No Hit
ATGTATACTGGTACCAGCAGTTCCCAGGCACGGCCCCCAGACTCCTCATT9160.13203698765396507No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9120.13146040692185168No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9050.13045139064065328No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC9050.13045139064065328No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9020.13001895509156822No Hit
TTGGATGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGACCAAACTGATCGTCCTGGGTCAGCCCAAGGCTGCC8990.12958651954248318No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8800.1268477610649446No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC8730.12583874478374618No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8660.12482972850254775No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT8650.12468558331951941No Hit
GATTAGTCCCATAAATGAGGAGTCTGGGGGCCGTGCCTGGGAACTGCTGG8630.12439729295346273No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG8600.12396485740437768No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8500.12252340557409422No Hit
AATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGG8290.11949635673049895No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8290.11949635673049895No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8200.11819905008324384No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8030.11574858197176195No Hit
GACCCAGGACGATCAGTTTGGTCCCTCCGCCGAACACCCAACTAGTCAGA7980.11502785605662022No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7960.11473956569056354No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7870.11344225904330842No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7820.11272153312816668No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7800.11243324276211No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7800.11243324276211No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA7770.11200080721302495No Hit
GTCCGAGGATGAGGCTGATTATTATTGTGCAACATTGGATGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGAC7670.11055935538274149No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG7510.10825303245428795No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC7380.10637914507491947No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG7300.10522598361069269No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7220.10407282214646592No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA7210.10392867696343758No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAG7170.10335209623132419No Hit
GTTCCCAGGCACGGCCCCCAGACTCCTCATTTATGGGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCT7080.10205478958406908No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG7070.10191064440104074No Hit
GGACCAAACTGATCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG7040.10147820885195569No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC6950.10018090220470056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1700.046.4657061
GGTATCA5000.043.9605981
GTATCAA11850.038.83651
TGGTATC2150.038.334972
AATGTTG11600.036.97057770
TATCAAC13150.034.9946252
ATCAACG14500.031.9710453
GTATAGT553.343256E-431.2220151
CAACGCA14750.031.196355
TCAACGC14750.031.196354
GTGCTAA450.00472488630.5281941
AACGCAG15200.030.2727746
AGAGTAC16000.028.77988211
CGCAGAG16300.028.4548538
CAGAGTA16450.027.99258810
GCAGAGT17000.027.4912289
TATAGTA500.00790827727.4733942
ACGCAGA16950.027.3518317
GAGTACT3450.025.89753512
AGTACTT3350.025.64480413
Produced by FastQC (version 0.11.5)