FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812784
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCGCGGCGGTCATAGAGTTCACCTTCAGGGAGA25000.30758479497627905No Hit
GTATATTACTGTGCGAGAGGCGAGACCTGGATAGCACCTCGTTCGTACTACTTTGACTACTGGGGCCAGGGAAAC15810.19451662434299888No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15430.18984133545935944No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14330.17630760448040314No Hit
GTCTATGGTGGGTCTTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC13480.1658497214512097No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13000.1599440933876651No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11390.14013563259119274No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10950.1347221401996102No Hit
AGTTTACACTGGTACCAACAGAAACCAGGTCAGTCTCCAAAGCTCCTCAT10290.12660190161223647No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10230.1258636981042934No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9600.11811256127089115No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9420.11589795074706194No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9340.11491367940313786No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8780.1080237799956692No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8750.10765467824169768No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8560.10531703379987796No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8420.10359455894801078No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8280.10187208409614362No Hit
ACCTATTACTGTCTTCAGAGTAGTAGTTTACCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA8220.10113388058820055No Hit
GGATAGCACCTCGTTCGTACTACTTTGACTACTGGGGCCAGGGAAACCTG8140.10014960924427646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4100.046.2490581
GTATCAA11400.043.5493551
TACTGAA8600.037.91023670
TATCAAC13750.036.07752
CAACGCA14400.034.4495
ATCAACG14650.033.861133
TCAACGC14850.033.405094
AACGCAG15100.033.3083046
CGCAGAG15950.031.3172748
AGAGTAC16300.030.43347211
ACGCAGA17000.029.382977
GCAGAGT17150.029.3268459
ATGTGTA1452.3646862E-1128.50951611
AATATAC7000.028.0513293
TATAGTC500.007789633727.55922
GAACCGT756.2237144E-527.5591986
TAATATA7300.027.3704382
TGGATTG6550.027.23552770
TGTATAA1555.2750693E-1126.67019314
GTGTATA1555.2750693E-1126.67019313
Produced by FastQC (version 0.11.5)