FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924704
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT10260.11095442433470602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.040.078651
GTATCAA37650.039.0338821
GTGGTAT5750.036.4271281
TATCAAC42950.034.4383352
TGGTATC6100.033.7558822
TCGTATG2450.032.5571140
AGGGGGG4000.032.1104570
TCAACGC47050.031.5832144
ATCAACG47250.031.2316343
CAACGCA48050.030.8544925
CGTAATT800.004683749830.58138370
AACGCAG48600.030.5759326
AGACGTA3100.029.90646225
CTCGTAT2150.029.01097539
CTTATAC3100.028.7988151
TAACGTA754.7256894E-528.69609867
ACGCAGA51700.028.6761767
AGAGTAC50600.028.28222811
CAGAGTA51250.028.2583410
TTATACA3300.028.0787582
Produced by FastQC (version 0.11.5)