FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330699
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22130.6691885974859313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCAG150.002402761768.64455422
TTGGATT200.00750612951.48341822
GGTAAGC200.0075286351.4444581
TAAGCAT200.007551181651.4055633
GTATCAA19500.040.4520531
CTCAGAT451.0336568E-438.11854620
GTGGTAT3450.037.7756421
TATCAAC21300.037.005572
AACGCAG23000.034.419386
TGGTATC3800.034.270382
GGTATCA7750.033.6325071
CAGAGTA22950.033.46932610
AGAGTAC22900.033.39266211
GCAGAGT23900.032.7128689
CAACGCA24250.032.6451845
ACGCAGA24450.032.3879437
ATCAACG24400.032.3040433
CGCAGAG24500.032.046858
TCAACGC24900.031.930634
TACCACT450.004760734730.4763818
Produced by FastQC (version 0.11.5)