FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537611
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT14600.2715718242372273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATACG200.00749772151.5024411
GTATCAA20700.041.652581
TACTAGT350.001204087740.3392362
AGGGGGG3850.039.27831370
TATACAC2600.036.9761123
CTCTTAT952.2919266E-1036.158881
TATCAAC24100.035.9021152
GGTATCA7950.034.566981
TATAGCT400.002656853534.334964
TTATACA2850.033.7325942
TCGTATG2800.033.4540840
AACGCAG26700.032.7918176
TACACAT2950.032.5891155
CAACGCA26800.032.5413445
ATCAACG26900.032.4203723
CTTATAC2900.031.9819051
TCAACGC27300.031.9453494
AGAGTAC27250.031.62596311
CTCGTAT2750.031.50958839
CAGAGTA27600.031.10050810
Produced by FastQC (version 0.11.5)