FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603809
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT10950.1813487377631006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1650.048.3482240
CTCGTAT1550.046.9330139
CTTATAC2150.043.059271
AGGGGGG2200.042.37704570
GTATCAA23850.041.8355941
ACGCGTA1850.040.80866227
GGTATCA9500.035.7316361
GTGGTAT3550.034.7708631
TATCAAC28600.034.7415772
TCTCGTA2000.034.60047538
GTAATCT2100.034.45927434
TTATACA2700.034.262382
GTAGTAA2450.033.68588631
CAACGCA29650.033.626825
GCGTAGT2350.033.6057529
TCAACGC29800.033.3425874
TATACAC2700.032.99343
ATCAACG30250.032.9598433
AACGCAG30450.032.743366
CGTATGC2450.032.6212141
Produced by FastQC (version 0.11.5)