FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603809
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21210.35127002081784137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28950.038.797371
GTGGTAT5550.037.0199051
TGGTATC6250.034.500212
TATCAAC33650.033.463552
AACGCAG34750.032.1087956
ATCAACG35550.031.4825123
CAACGCA35600.031.3421525
TCAACGC35600.031.3421524
GGTATCA12800.031.3006211
CAGAGTA35700.030.50000810
ACGCAGA36500.030.4780857
AGAGTAC35750.030.26579511
CGCAGAG37050.030.0306198
GCAGAGT37500.029.6702529
TACGGAC605.677617E-428.5222
ACGGGTC2850.025.22989816
AGTACGG36800.024.84302713
GAGTACG37300.024.51001212
ATATATC700.00139641124.4474283
TACGGGT7750.024.29775215
Produced by FastQC (version 0.11.5)